The interaction of transferrin with isolated hepatocytes.
The interaction of transferrin with isolated hepatocytes.
复制标题
转铁蛋白与分离的肝细胞的相互作用。
DOI：
10.1016/0304-4165(80)90400-6
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Aisen,P
中科院分区：
文献类型：
--
作者：
Young,SP;Aisen,P
新鲜分离的大鼠肝细胞显示出约 36 700 个高亲和力位点，二铁转铁蛋白可以与二铁转铁蛋白结合，表观关联常数为 1.62·1071·mol−1。肝细胞从二铁转铁蛋白中吸收铁与时间呈线性关系，并且随着不同转铁蛋白浓度的增加而加速。脱铁转铁蛋白能够通过不依赖于所使用的脱铁转铁蛋白浓度的过程来减少肝细胞从二铁转铁蛋白中吸收的净铁。细胞吸收铁的速率。在这些孵育过程中，脱铁转铁蛋白的免疫沉淀表明铁从细胞释放到脱铁转铁蛋白，同时铁从二铁转铁蛋白中被吸收。铁的同时摄取和释放以及对脱铁转铁蛋白浓度的不敏感性表明铁的摄取和释放过程通过单独的机制发生。脱铁转铁蛋白对铁净保留的影响可能是控制铁在体内储存和利用位点之间分布的一种方式。
Freshly isolated rat heptocytes display about 36 700 high-affinity sites to which deferric transferrin may bind with an apparent association constant of 1.62·1071·mol−1.Uptake of iron from diferric transferrin by hepatocytes is linear with time and is accelerated at increased differric transferrin concentrations.Apotransferrin is able to decrease net iron uptake by hepatocytes from diferric transferrin by a process not dependent on the apotransferrin concentrations used or on the rate at which the cells take up iron. Immunoprecipitation of the apotransferrin during these incubations indicates that iron is being released from the cells to apotransferrin at the same time as iron is being taken up from diferric transferrin. The simultaneous uptake and release of iron, and the insensitivity to apotransferrin concentration, suggest that the processes of iron uptake and release occur via separate mechanisms. The effect of apotransferrin on net retention of iron may be one way in which the in vivo distribution of iron between sites of storage and utilization is controlled.