Growth factors derived from a human malignant glioma cell line, U-251MG.
Growth factors derived from a human malignant glioma cell line, U-251MG.
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源自人类恶性神经胶质瘤细胞系 U-251MG 的生长因子。
DOI：
10.1007/bf00172915
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发表时间：
1989
影响因子：
3.9
通讯作者：
Gillespie,GY
中科院分区：
文献类型：
--
作者：
Kuratsu,J;Estes,JE;Yokota,S;MahaleyJr,MS;Gillespie,GY
在无血清条件下培养的人恶性胶质瘤细胞系U-251 Mg显示产生BALB/c 3T3细胞的生长因子（胶质瘤源性生长因子-1，GDGF-1）。GDGF-1的生物活性存在于一个耐热耐酸蛋白中，凝胶渗透色谱法估计其分子量为25 kDa。GDGF-1活性被山羊抗人血小板衍生生长因子（PDGF）抗体中和，表明两者具有免疫学相关性。U-251 Mg细胞组成性表达c-sis mRNA。当细菌脂多糖刺激U-251 Mg细胞时，除了pdgf样物质外，还产生2种新的生长因子（GDGF-2和GDGF-3）。测定GDGF-2为100 kDa - MW，不被山羊抗pdgf抗血清中和。GDGF-3的生物活性也具有耐热性和耐酸性，明显为14 kDa MW，与PDGF没有任何共同的抗原性。GDGF-2和GDGF-3目前正在调查中，关于它们性质的证据将在其他地方公布。我们对这种胶质瘤细胞系的研究结果进一步证明，生长因子相关基因的不适当表达可能在导致恶性转化和/或不受控制的增殖过程中发挥重要的自分泌作用，并可能为胶质瘤新生血管等过程提供旁分泌刺激。
A human malignant glioma cell line, U-251 Mg, cultured under serum free conditions, was shown to produce a growth factor for BALB/c 3T3 cells (glioma-derived growth factor-1, GDGF-1). The biological activity of GDGF-1 resided in a heat- and acid-resistant protein with a molecular weight (MW) of 25 kDa estimated by gel permeation chromatography. GDGF-1 activity was neutralized by a goat anti-human platelet derived growth factor (PDGF) antibody, indicating that the two factors were immunologically related. Furthermore, U-251 Mg cells constitutively expressed c-sis mRNA. When U-251 Mg cells were stimulated with bacterial lipopolysaccharide, 2 novel growth factors (GDGF-2 and GDGF-3) were produced in addition to the PDGF-like substance. GDGF-2 was determined to be >100 kDa MW and was not neutralized by the goat anti-PDGF antiserum. The biological activity of GDGF-3 was also heat- and acid- resistant with an apparent 14 kDa MW This factor also did not show any common antigenicity with PDGF. GDGF-2 and GDGF-3 are currently under investigation and evidence as to their natures will be published elsewhere. Our findings with this glioma cell line provide further evidence that inappropriate expression of growth factor-related genes could play important autocrine role(s) in the processes leading to malignant transformation and/or uncontrolled proliferation and may provide a paracrine stimulus for such processes as glioma neovascularization.