Differences in interleukin-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages
Differences in interleukin-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages
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DOI：
10.1016/j.archoralbio.2018.06.004
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发表时间：
2018-09-01
影响因子：
3
通讯作者：
Shibata, Ken-ichiro
中科院分区：
文献类型：
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作者：
Hasebe, Akira;Saeki, Ayumi;Shibata, Ken-ichiro
目的：探讨白色念珠菌对树突状细胞和巨噬细胞IL-1 β释放诱导活性的机制差异，因为IL-1 β是宿主防御念珠菌病的重要促炎细胞因子之一。设计：本研究采用两株白色念珠菌。一种菌株是尿苷-营养不良（CAI4），需要尿苷来生长和形成菌丝，另一种菌株是没有任何特定的营养不良（pACT1-GFP），通过血清成分培养自然形成菌丝。小鼠巨噬细胞和树突状细胞系先用LPS引物，然后用白色念珠菌CAI4或pACT1-GFP刺激。结果：两株白色念珠菌均诱导树突状细胞释放IL-1 β，白色念珠菌pACT1-GFP诱导巨噬细胞释放IL-1 β，而CAI4诱导少量。这些差异被认为是由于白色念珠菌CAI4或pACT1-GFP诱导的细胞培养上清中细胞外ATP释放量的差异。为了诱导巨噬细胞和树突状细胞释放IL-1 β，需要白色念珠菌与细胞直接接触。此外，巨噬细胞需要白色念珠菌从酵母到菌丝的形态变化来诱导IL-1 β的释放，而树突状细胞不需要。死亡的白色念珠菌可诱导树突状细胞释放IL-1 β，但不能诱导巨噬细胞释放IL-1 β。结论：白色念珠菌诱导树突状细胞和巨噬细胞释放IL-1 β的机制不同。
Objective: The purpose of this study is to elucidate differences in the mechanism of the IL-1 beta release-inducing activity of Candida albicans toward dendritic cells and macrophages because IL-1 beta is one of the proinflammatory cytokines which is crucial in host defense against candidiasis.Design: Two C. albicans strains were used in this study. One strain is uridine-auxotrophic (CAI4) that needs uridine to grow and form hyphae, and another is a strain without any specific auxotrophy (pACT1-GFP), which forms hyphae naturally by culturing with serum components. Murine macrophage and dendritic cell lines were primed with LPS and then stimulated with C. albicans CAI4 or pACT1-GFP.Results: Both strains of C. albicans induced IL-1 beta release from dendritic cells, and C. albicans pACT1-GFP induced IL-1 beta release but CAI4 induced little amounts in macrophages. These differences were suggested to be due to the difference in the amount of extracellular ATP released in the cell culture supernatants induced by C. albicans CAI4 or pACT1-GFP. For induction of IL-1 beta release from both macrophages and dendritic cells by C. albicans, direct contacts of the microbes with cells were required. In addition, macrophages required morphological change of C. albicans from yeast to hyphae for induction of IL-1 beta release, whereas dendritic cells did not require it. Dead C. albicans could induce IL-1 beta release from dendritic cells, but could not from macrophages.Conclusions: There are different mechanisms by which C. albicans induces IL-1 beta release from dendritic cells and macrophages.