Alterations of the M6p/Igf2 receptor gene in lung adenocarcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine in rats
Alterations of the M6p/Igf2 receptor gene in lung adenocarcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine in rats
复制标题
N-亚硝基双(2-羟丙基)胺诱导的大鼠肺腺癌中M6p/Igf2受体基因的改变
作者：
T. Tsujiuchi;Y. Sasaki;M. Tsutsumi;Y. Konishi
研究了N-亚硝基双（2-羟丙基）胺（BHP）诱导的大鼠肺腺癌中甘露糖6-磷酸/胰岛素样生长因子II受体（M6 p/lgf 2 r）基因的改变。雄性Wistar大鼠，6周龄，给予2000 ppm的BHP在其饮用水12周，并保持不进一步的治疗，直到杀死在第25周。共获得12例肺腺癌，并从每例中提取总RNA，分别通过逆转录（RT）-聚合酶链反应（PCR）单链构象多态性分析和RT-PCR分析评估M6 p/Igf 2 r基因的突变和异常转录水平。在外显子27、28、31、33和34中未发现突变。12例腺癌中有3例（25%）检测到nt 3698至4902、3366至4902和3817至4697缺失的异常转录本。这些结果表明，M6 p/Igf 2 r基因的改变可能参与BHP诱导的大鼠肺腺癌的发展。© 2002 Wiley利斯公司
Alterations of the mannose 6‐phosphate/insulin‐like growth factor II receptor (M6p/lgf2r) gene were investigated in lung adenocarcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine (BHP) in rats. Male Wistar rats, 6 wk old, were given 2000 ppm BHP in their drinking water for 12 wk and maintained without further treatment until killed at week 25. A total of 12 lung adenocarcinomas were obtained, and total RNAs were extracted from each for assessment of mutations and levels of aberrant transcripts of the M6p/Igf2r gene by reverse transcription (RT)–polymerase chain reaction (PCR) single‐strand conformation polymorphism analysis and RT‐PCR analysis, respectively. No mutations were found in exons 27, 28, 31, 33, and 34. Aberrant transcripts bearing deletions of nt 3698 to 4902, 3366 to 4902, and 3817 to 4697 were detected in three of 12 adenocarcinomas (25%). These results suggest that alterations of the M6p/Igf2r gene may be involved in the development of lung adenocarcinomas induced by BHP in rats. © 2002 Wiley‐Liss, Inc.
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胰岛素样生长因子 II/非阳离子依赖性甘露糖 6-磷酸受体和溶酶体酶识别。
影响因子：
3.9
作者：
Dahms,NM
通讯作者：
Dahms,NM
影响因子：
8
作者：
Hankins Gr;Denisov At;Rex C. Bentley;Patel Mr;Marks;Iglehart Jd;R. Jirtle
通讯作者：
Hankins Gr;Denisov At;Rex C. Bentley;Patel Mr;Marks;Iglehart Jd;R. Jirtle
DOI：
10.1073/pnas.88.2.580
发表时间：
1991-01-01
影响因子：
11.1
作者：
DENNIS, PA;RIFKIN, DB
通讯作者：
RIFKIN, DB
影响因子：
8
作者：
A. T. D. Souza;G. R. Hankins;M. Washington;R. Fine;T. Orton;R. Jirtle
通讯作者：
A. T. D. Souza;G. R. Hankins;M. Washington;R. Fine;T. Orton;R. Jirtle