Homozygous deletions of 9p21 in primary human bladder tumors detected by comparative multiplex polymerase chain reaction.
Homozygous deletions of 9p21 in primary human bladder tumors detected by comparative multiplex polymerase chain reaction.
复制标题
通过比较多重聚合酶链反应检测原发性人膀胱肿瘤中 9p21 的纯合缺失。
作者：
P. Cairns;K. Tokino;Yolanda Eby;D. Sidransky
对原发膀胱肿瘤的缺失作图研究发现，9号染色体的每条臂上都有不重叠的缺失区域，这表明该染色体上存在两个不同的抑癌基因。P臂上的缺失区域与先前报道的在多种肿瘤中丢失的9p区域重叠。使用12个微卫星标记对112例原发膀胱肿瘤的9P杂合性丢失进行了详细分析，发现在9p21-22的α-干扰素基因座周围有最小的丢失区域。然后，通过一种新的、可比较的、多重聚合酶链式反应分析，在这些肿瘤中发现了频繁的α-干扰素纯合子缺失，并随后通过Southern分析进行了证实。基于这些缺失，一个可能与膀胱肿瘤发生有关的抑癌基因被定位在10 cM的区域(位于D9S162和D9S171的两侧)，这是以前与许多肿瘤的进展有关的基因。多重聚合酶链式反应分析的应用将使快速鉴定许多肿瘤中的纯合子缺失，从而有助于对关键抑制基因的定位研究。
Deletion mapping studies of primary bladder tumors have identified nonoverlapping areas of loss on each arm of chromosome 9, indicating that two distinct tumor suppressor loci are located on this chromosome. The deleted region on the p arm overlaps an area of 9p previously reported to be lost in a variety of neoplasms. Detailed loss of heterozygosity analysis of 9p in 112 primary bladder tumors using 12 microsatellite markers identified a minimal area of loss around the alpha-interferon locus at 9p21-22. Frequent homozygous deletions of the alpha-interferon locus were then identified in these tumors by a novel, comparative, multiplex polymerase chain reaction assay and were subsequently confirmed by Southern analysis. Based on these deletions, a putative tumor suppressor gene locus involved in bladder tumorigenesis was localized to a 10 cM region (flanked by D9S162 and D9S171), previously implicated in the progression of many neoplasms. Application of the multiplex polymerase chain reaction-based assay will allow rapid identification of homozygous deletions in many neoplasms and thus aid in mapping studies of critical suppressor genes.
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DOI：
10.1073/pnas.89.21.10557
发表时间：
1992-11-01
影响因子：
11.1
作者：
FOUNTAIN, JW;KARAYIORGOU, M;DRACOPOLI, NC
通讯作者：
DRACOPOLI, NC
影响因子：
56.9
作者：
FEARON, ER;CHO, KR;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
56.9
作者：
SIDRANSKY, D;VONESCHENBACH, A;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
9 号染色体在人类膀胱癌中的作用。
影响因子：
11.2
作者：
Miyao,N;Tsai,YC;Lerner,SP;Olumi,AF;Spruck3rd,CH;Gonzalez-Zulueta,M;Nichols,PW;Skinner,DG;Jones,PA
通讯作者：
Jones,PA
浅表和局部晚期人类膀胱癌的分子遗传改变。
影响因子：
11.2
作者：
PrestiJr,JC;Reuter,VE;Galan,T;Fair,WR;Cordon-Cardo,C
通讯作者：
Cordon-Cardo,C