Characterization of a Novel Periodontal Ligament-specific Periostin Isoform
Characterization of a Novel Periodontal Ligament-specific Periostin Isoform
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DOI：
10.1177/0022034514543015
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发表时间：
2014-07
影响因子：
7.6
通讯作者：
S. Yamada;T. Tauchi;T. Awata;Kenichiro Maeda;T. Kajikawa;M. Yanagita;S. Murakami
中科院分区：
文献类型：
--
作者：
S. Yamada;T. Tauchi;T. Awata;Kenichiro Maeda;T. Kajikawa;M. Yanagita;S. Murakami
骨膜蛋白是一种间充质细胞标志物，主要在富含胶原的纤维结缔组织中表达，包括心脏瓣膜、肌腱、软骨膜、骨膜和牙周韧带（PDL）。在小鼠中，骨膜蛋白表达的敲低导致早发性牙周炎和急性心肌梗死后心脏愈合失败，表明骨膜蛋白对于结缔组织稳态和再生是必不可少的。然而，其在牙周组织中的作用尚未完全确定。在这项研究中，我们描述了一种新的人类同种型骨膜蛋白（PDL-POSTIN）。通过逆转录聚合酶链反应（RT-PCR）进行的异构体特异性分析显示，PDL-POX 4主要在PDL中表达，在其他组织和器官中的表达要低得多。与转染全长骨膜蛋白同种型的细胞相比，转染PDL-POST 3的PDL细胞系显示出增强的碱性磷酸酶（ALST 3）活性和钙化结节形成。针对整合素-αv的中和抗体可抑制转染PDL-P03的细胞中的ALP 03活性和钙化结节形成。此外，免疫共沉淀试验显示，PDL-POl与整联蛋白αvβ3的结合比骨膜蛋白的常见同种型更强，导致整联蛋白αvβ3-粘着斑激酶（FAK）信号通路的强烈激活。这些结果表明，PDL-PO通过整合素αvβ3正向调节PDL细胞的细胞分化和矿化。
Periostin is a mesenchymal cell marker predominantly expressed in collagen-rich fibrous connective tissues, including heart valves, tendons, perichondrium, periosteum, and periodontal ligament (PDL). Knockdown of periostin expression in mice results in early-onset periodontitis and failure of cardiac healing after acute myocardial infarction, suggesting that periostin is essential for connective tissue homeostasis and regeneration. However, its role(s) in periodontal tissues has not yet been fully defined. In this study, we describe a novel human isoform of periostin (PDL-POSTN). Isoform-specific analysis by reverse-transcription polymerase chain-reaction (RT-PCR) revealed that PDL-POSTN was predominantly expressed in the PDL, with much lower expression in other tissues and organs. A PDL cell line transfected with PDL-POSTN showed enhanced alkaline phosphatase (ALPase) activity and calcified nodule formation, compared with cells transfected with the full-length periostin isoform. A neutralizing antibody against integrin-αv inhibited both ALPase activity and calcified nodule formation in cells transfected with PDL-POSTN. Furthermore, co-immunoprecipitation assays revealed that PDL-POSTN bound to integrin αvβ3 more strongly than the common isoform of periostin, resulting in strong activation of the integrin αvβ3-focal adhesion kinase (FAK) signaling pathway. These results suggest that PDL-POSTN positively regulates cytodifferentiation and mineralization in PDL cells through integrin αvβ3.