Association of a protein phosphatase 1 activity with the human factor C1 (HCF) complex
Association of a protein phosphatase 1 activity with the human factor C1 (HCF) complex
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DOI：
10.1093/nar/28.3.678
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发表时间：
2000-02-01
影响因子：
14.9
通讯作者：
Lamond, AI
中科院分区：
文献类型：
--
作者：
Ajuh, PM;Browne, GJ;Lamond, AI
我们已经筛选了一个人的cDNA表达文库与地高辛标记的蛋白磷酸酶1（PP1）探针，以确定新的PP1相互作用的蛋白。11个cDNA克隆被分离，其中包括编码两个以前的特点和六个新的PP1结合蛋白的基因。其中三个cDNA编码一种称为宿主细胞因子（HCF）的蛋白质，该蛋白质是单纯疱疹病毒（HSV）立即早期（IE）基因转录所需的细胞复合物的重要组成部分。我们证明了HCF和PP 1在核提取物中以复合物的形式存在，并且这种复合物不同于与HSV VP 16相关的HCF形式。数据表明HCF和PP 1的新作用，这可能与它们在转录和细胞周期进程中的功能有关。
We have screened a human cDNA expression library with a digoxygenin-labelled protein phosphatase 1 (PP1) probe to identify novel PP1 interacting proteins. Eleven cDNA clones were isolated, which included genes encoding two previously characterised and six novel PP1 binding proteins. Three of the cDNAs encoded a protein called host cell factor (HCF), which is an essential component of the cellular complex required for the transcription of the herpes simplex virus (HSV) immediate-early (IE) genes. We demonstrate that HCF and PP1 exist as a complex in nuclear extracts and that this complex is distinct from the form of HCF that associates with HSV VP16, The data suggest novel roles for HCF and PP1, which may be relevant to their functions in transcription and cell cycle progression.