Human papillomavirus detection and typing in thin prep cervical cytologic specimens comparing the Digene Hybrid Capture II Assay, the Roche Linear Array HPV Genotyping Assay, and the Kurabo Gene Square Microarray Assay
Human papillomavirus detection and typing in thin prep cervical cytologic specimens comparing the Digene Hybrid Capture II Assay, the Roche Linear Array HPV Genotyping Assay, and the Kurabo Gene Square Microarray Assay
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DOI：
10.1016/j.jviromet.2010.07.016
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发表时间：
2010-10-01
影响因子：
3.1
通讯作者：
Brown, D.
中科院分区：
文献类型：
--
作者：
Ermel, A.;Qadadri, B.;Brown, D.
比较了三种检测宫颈细胞学标本中HPV DNA的方法：Digene Hybrid Capture II Assay（HC）、Roche Linear Array HPV Genotyping Assay（LA）和Kurabo GeneSquare Microarray（GS）。该研究的主要目的是将细胞学与HPV检测相关联，并确定HPV检测的检测对之间的一致性。进行薄层巴氏涂片，并通过HC、IA和GS检测上清液。对于在LA检测试剂盒中与HPV 52/33/35/58探针反应的标本，对HPV 52、33、35或58型进行型特异性PCR。计算二项式比例和kappa系数，以确定试验之间的一致性。可获得202例受试者的细胞学结果和上清液。在所有三种检测中，HPV检测随着细胞学异常的恶化而增加。对于所有细胞学组，LA和GS检测到更多的HPV（所有和致癌）比HC。然而，对于所有三种检测方法中代表的致癌HPV类型的检测，检测方法之间的差异不太明显。LA和GS之间的一致性最高。在LA检测试剂盒中与HPV 52/33/35/58探针反应但使用制造商提供的算法认为HPV 52-LA阴性的12份标本中，有4份使用型别特异性HPV 52 PCR确认存在HPV 52。所有这四份标本对HPV 52也呈GS阳性。(C)2010爱思唯尔有限公司版权所有。
Three methods for the detection of HPV DNA were compared in cervical cytologic specimens: the Digene Hybrid Capture II Assay (HC), the Roche Linear Array HPV Genotyping Assay (LA) and the Kurabo GeneSquare Microarray (GS). The main goals of the study were to correlate cytology with HPV detection and to determine agreement between assay pairs for HPV detection. Thin-prep Pap smears were performed and supernates were tested by HC, IA, and GS. For specimens reacting with the HPV 52/33/35/58 probe in the LA assay, type-specific PCR was performed for HPV types 52, 33, 35, or 58. Binomial proportions and kappa coefficients were calculated for agreement between assays. Cytology results and supernatant were available for 202 subjects. HPV detection increased with worsening cytologic abnormality in all three assays. For all cytologic groups, LA and GS detected more HPV (all and oncogenic) than HC. However, for detection of oncogenic HPV types represented in all three assays, differences between assays were less pronounced. The highest agreement was between LA and GS. In four of 12 specimens reacting with the HPV 52/33/35/58 probe in the LA assay but deemed HPV 52-LA-negative using an algorithm provided by the manufacturer, the presence of HPV 52 was confirmed using type-specific HPV 52 PCR. All four of these specimens were also GS-positive for HPV 52. (C) 2010 Elsevier B.V. All rights reserved.