Selecting, Acquiring, and Using Small Molecule Libraries for High-Throughput Screening.
Selecting, Acquiring, and Using Small Molecule Libraries for High-Throughput Screening.
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选择、获取和使用小分子文库进行高通量筛选。
DOI：
10.1002/9780470559277.ch110252
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发表时间：
2012
影响因子：
--
通讯作者：
Thomas,CraigJ
中科院分区：
文献类型：
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作者：
Dandapani,Sivaraman;Rosse,Gerard;Southall,Noel;Salvino,JosephM;Thomas,CraigJ
选择、获取和使用高质量的小分子文库进行筛选是药物发现和化学生物学项目的一个重要方面。随着研究人员对特定生物靶点、过程和环境的小分子适用性的进一步了解，筛选文库也在不断发展。围绕任何给定的小分子库的组成的决定都是由许多变量决定的，并且对最佳实践的意见也各不相同。任何收集的适应性都依赖于预先过滤，以避免有问题的化合物，评估适当的物理化学性质，安装理想的结构独特性水平，并确定所需的分子复杂性程度。这些标准在不断的评估和修订中，因为学术和工业组织寻求从他们的筛选组合中产生不断改进的结果。实际问题包括成本，化合物管理，筛选复杂程度和分析目的也在文库组成的选择中发挥重要作用。本综述试图为所有从事小分子筛选的组织提供建议，这些建议是基于当前图书馆选择和获取的最佳实践和理论考虑。Protoc。化学。《圣经》4:177‐191©2012 by John Wiley & Sons， Inc。
The selection, acquisition, and use of high‐quality small molecule libraries for screening is an essential aspect of drug discovery and chemical biology programs. Screening libraries continue to evolve as researchers gain a greater appreciation of the suitability of small molecules for specific biological targets, processes, and environments. The decision surrounding the makeup of any given small molecule library is informed by a multitude of variables, and opinions vary on best practices. The fitness of any collection relies upon upfront filtering to avoid problematic compounds, assess appropriate physicochemical properties, install the ideal level of structural uniqueness, and determine the desired extent of molecular complexity. These criteria are under constant evaluation and revision as academic and industrial organizations seek out collections that yield ever‐improving results from their screening portfolios. Practical questions including cost, compound management, screening sophistication, and assay objective also play a significant role in the choice of library composition. This overview attempts to offer advice to all organizations engaged in small molecule screening based upon current best practices and theoretical considerations in library selection and acquisition.Curr. Protoc. Chem. Biol. 4:177‐191 © 2012 by John Wiley & Sons, Inc.