Expression of Genes for Appetite-Regulating Peptides in the Hypothalamus of Genetically Selected Lean and Fat Sheep
Expression of Genes for Appetite-Regulating Peptides in the Hypothalamus of Genetically Selected Lean and Fat Sheep
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DOI：
10.1159/000262283
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发表时间：
2010-01-01
影响因子：
4.1
通讯作者：
Clarke, Iain J.
中科院分区：
文献类型：
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作者：
Anukulkitch, Chantacha;Rao, Alexandra;Clarke, Iain J.
背景/目标：其目的是确定是否可以解释下丘脑食欲调节肽基因表达水平的变化，绵羊的身体组成的遗传选择。我们研究了基因表达的遗传瘦，正常和脂肪母羊（n = 5/组）的下丘脑。方法：测定血浆生长激素（GH）和代谢指标，用原位杂交技术定量脑组织基因表达。结果如下：各组之间的体重和自愿摄食量（VFI）相似，但瘦和胖动物分别具有低和高的肥胖指数。GH水平在瘦和胖动物高于对照组。在弓状核（ARC），神经肽Y（NPY）的表达/细胞在瘦动物高于正常动物，但整体NPY的表达是相似的脂肪和正常动物。各组ARC中的前阿黑皮素（POMC）和瘦素受体（ObRb）表达相似。食欲素（ORX）和黑色素浓集激素（MCH）的表达与肥胖呈负相关，在瘦动物中较高，在胖动物中较低。结论：促食欲神经肽的基因表达以一致的方式改变。能量消耗通过MCH减少，但通过ORX增加，因此后者的表达增加可能导致瘦动物中能量消耗增加，反之亦然。版权所有（C）2009 S. Karger AG，巴塞尔
Background/Aims: The aim was to determine whether genetic selection of sheep for body composition could be accounted for by changes in the level of expression of genes for appetite-regulating peptides in the hypothalamus. We examined gene expression in the hypothalamus of genetically lean, normal and fat ewes (n = 5/group). Methods: Plasma growth hormone (GH) and metabolic indicators were measured and gene expression in brains was quantified by in situ hybridization. Results: Body weight and voluntary food intake (VFI) were similar between groups, but lean and fat animals respectively had low and high indices of adiposity. GH levels were higher in lean and fat animals than in controls. In the arcuate nucleus (ARC), neuropeptide Y (NPY) expression/cell was higher in the lean animals than in normal animals, but overall NPY expression was similar in fat and normal animals. Pro-opiomelanocortin (POMC) and leptin receptor (ObRb) expression in the ARC was similar across groups. Orexin (ORX) and melanin-concentrating hormone (MCH) expression was inversely correlated to adiposity, being higher in lean and lower in fat animals. Conclusion: Expression of genes for orexigenic neuropeptides is altered in a consistent way. Energy expenditure is reduced by MCH but increased by ORX, so increased expression of the latter may cause increased energy expenditure in the lean animals and vice versa in the fat animals. Copyright (C) 2009 S. Karger AG, Basel