Use of digital aroma technology and SPME GC-MS to compare volatile compounds produced by bacteria isolated from processed poultry†
Use of digital aroma technology and SPME GC-MS to compare volatile compounds produced by bacteria isolated from processed poultry†
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DOI：
10.1002/(sici)1097-0010(199811)78:3
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发表时间：
1998-11
影响因子：
4.1
通讯作者：
J. W. Arnold;S. Senter
中科院分区：
文献类型：
--
作者：
J. W. Arnold;S. Senter
采用数字香气技术、固相微萃取（SPME）和气相色谱质谱（GC-MS）分析顶空挥发性有机物，比较了家禽加工环境中对食品安全重要的细菌种类和生物膜中常见的细菌种类。数字芳香技术的仪器，称为电子鼻，测量由样品顶空挥发性气体引起的聚合物传感器电阻的变化。Sammon映射技术的图形输出产生了样本之间差异或相似的模式。使用人工神经网络软件对样本组进行建模，并对后续未知数进行分类。用聚二甲基硅氧烷SPME纤维从密封培养物顶空分离出的化合物经气相色谱-质谱分析鉴定主要为醇类和吲哚类。这些定性特征对于特定生物的纯度和培养的可重复性是重复的，因物种而异，并被用作比较电子鼻图形输出的客观标准。©1998化学工业协会。
Digital aroma technology, solid-phase micro-extraction (SPME) and gas chromatographic mass spectral (GC-MS) analysis of the headspace volatile organic compounds were used to compare bacterial species important for food safety and common to biofilms in the poultry processing environment. The instrument for digital aroma technology, called the electronic nose, measured changes in resistance of polymer sensors caused by volatile gases from the headspace of samples. Graphical output by the Sammon mapping technique produced patterns of differences or similarities among the samples. Artificial neural network software was used to model groups of samples and classify subsequent unknowns. Compounds isolated from the headspace of sealed cultures using polydimethylsiloxane SPME fibres and identified by GC-MS analyses were predominantly alcohols and indole. These qualitative profiles were repetitive for specific organisms in relation to purity and repeatability of the cultures, differed by species and were used as objective standards to compare the graphical outputs of the electronic nose. © 1998 Society of Chemical Industry.