Nucleotide sequence and transcription of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum
Nucleotide sequence and transcription of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum
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烟草 32,000 道尔顿类囊体膜蛋白基因的核苷酸序列和转录
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
M. Sugiura
中科院分区：
文献类型：
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作者：
M. Sugita;M. Sugiura
摘要测定了烟叶32000道尔顿类囊体膜蛋白基因的核苷酸序列。该基因位于叶绿体DNA大单拷贝区右边缘距反向重复535个碱基对（bp）处。编码区包含1059bp（353个密码子）。推导出的氨基酸序列与菠菜和烟叶的氨基酸序列相同。该基因的转录从ATG密码子上游85 bp开始，在TAA停止密码子下游91-95 bp处终止。32,000道尔顿蛋白基因的启动子序列与核酮糖-1,5-二磷酸羧化酶的大亚基具有很强的同源性。
SummaryThe nucleotide sequence of the gene for the 32,000 dalton thylakoid membrane protein from Nicotiana tabacum has been determined. This gene is located 535 base pairs (bp) away from the inverted repeat on the right margin of the large single-copy region of the chloroplast DNA. The coding region contains 1059 bp (353 codons). The deduced amino acid sequence is identical with those of spinach and Nicotiana debneyi. Transcription of this gene starts 85 bp upstream from the ATG codon and terminates 91–95 bp downstream from the TAA stop codon. A strong homology was observed in the promoter sequences between the genes for the 32,000 dalton protein and the large subunit of ribulose-1,5-bisphosphate carboxylase.