BIOSYNTHESIS OF RETICULOCYTE MEMBRANE PROTEINS BY MEMBRANE-FREE POLYRIBOSOMES - (RABBIT HEMOGLOBIN GEL ELECTROPHORESIS)
BIOSYNTHESIS OF RETICULOCYTE MEMBRANE PROTEINS BY MEMBRANE-FREE POLYRIBOSOMES - (RABBIT HEMOGLOBIN GEL ELECTROPHORESIS)
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DOI：
10.1073/pnas.70.5.1526
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
LODISH, HF
中科院分区：
文献类型：
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作者：
LODISH, HF
完整的兔网织红细胞合成两种主要种类的膜多肽；至少有10种网织红细胞膜多肽种类不是由细胞产生的。网织红细胞的无细胞提取物，不含任何膜或膜结合的多核糖体，合成大量的这两种膜多肽；这些多肽之一在掺入膜后似乎发生了修饰，可能是由于丢失了 20-40 个氨基酸。剥夺血红素会导致膜蛋白、球蛋白和所有其他细胞质蛋白裂解物的合成受到抑制。
Intact rabbit reticulocyte cells synthesize two predominant species of membrane polypeptides; at least 10 reticulocyte membrane polypeptide species are not produced by the cells. Cell-free extracts of reticulocytes, free of any membranes or membrane-bound polyribosomes, synthesize large amounts of these two membrane polypeptides; one of these polypeptides appears to be modified, probably by loss of 20-40 amino acids, after it is incorporated into the membrane. Deprivation of hemin results in inhibition of synthesis by lysates of membrane proteins, globin, and all other cytoplasmic proteins.