Genomic sequence and receptor for the Vibrio cholerae phage KSF-Iφ:: Evolutionary divergence among filamentous vibriophages mediating lateral gene transfer
Genomic sequence and receptor for the Vibrio cholerae phage KSF-Iφ:: Evolutionary divergence among filamentous vibriophages mediating lateral gene transfer
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DOI：
10.1128/jb.187.12.4095-4103.2005
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发表时间：
2005-06-01
影响因子：
3.2
通讯作者：
Mekalanos, JJ
中科院分区：
文献类型：
--
作者：
Faruque, SM;Bin Naser, I;Mekalanos, JJ
KSF-I Phi是一种新型的霍乱弧菌丝状噬菌体，通过异源DNA包装支持RS 1卫星噬菌体的形态发生，并促进基因的水平转移。我们分析了KSF-1 Phi感染的基因组序列、形态和受体，以及其与其他丝状弧菌噬菌体的系统发育关系。虽然携带编码甘露糖敏感血凝素（MSHA）IV型菌毛的mshA基因的菌株对KSF-1 Phi感染敏感，但天然存在的MSRA阴性菌株和mshA缺失突变株具有抗性。此外，D-甘露糖以及针对MSRA的单克隆抗体抑制MSHA阳性菌株被噬菌体感染，表明MSRA是KSF-1 Phi的受体。噬菌体基因组由7，107个核苷酸组成，含有14个开放阅读框，其中4个预测蛋白质产物与其他丝状噬菌体的蛋白质产物同源。虽然丝状菌的整体遗传组织似乎被保存在KSF-1 Φ，噬菌体的基因组序列不具有高水平的同一性与其他丝状菌，并揭示了一个高度嵌合结构。编码假定的复制蛋白，受体结合蛋白，和Zot样蛋白的10种不同的丝状弧菌噬菌体的基因组序列的单独的系统发育分析显示出不同的结果，这表明这些弧菌的进化涉及广泛的遗传物质的水平交换。以IV型皮利为受体的丝状真菌分属于不同的分支。虽然这些分支之一是由CTX Phi代表，它使用毒素共调节菌毛作为其受体，但至少有四个进化上分歧的基因共享一个共同的受体MSHA，并且这些基因中的大多数介导水平基因转移。由于MSHA存在于各种各样的霍乱弧菌菌株中，并被认为在环境中表达，因此使用该受体的各种丝状菌可能对霍乱弧菌的进化有显著贡献。
KSF-I Phi, a novel filamentous phage of Vibrio cholerae, supports morphogenesis of the RS1 satellite phage by heterologous DNA packaging and facilitates horizontal gene transfer. We analyzed the genomic sequence, morphology, and receptor for KSF-1 Phi infection, as well as its phylogenetic relationships with other filamentous vibriophages. While strains carrying the mshA gene encoding mannose-sensitive hemagglutinin (MSHA) type IV pilus were susceptible to KSF-1 Phi infection, naturally occurring MSRA-negative strains and an mshA deletion mutant were resistant. Furthermore, D-mannose as well as a monoclonal antibody against MSRA inhibited infection of MSHA-positive strains by the phage, suggesting that MSRA is the receptor for KSF-1 Phi. The phage genome comprises 7,107 nucleotides, containing 14 open reading frames, 4 of which have predicted protein products homologous to those of other filamentous phages. Although the overall genetic organization of filamentous phages appears to be preserved in KSF-1 Phi, the genomic sequence of the phage does not have a high level of identity with that of other filamentous phages and reveals a highly mosaic structure. Separate phylogenetic analysis of genomic sequences encoding putative replication proteins, receptor-binding proteins, and Zot-like proteins of 10 different filamentous vibriophages showed different results, suggesting that the evolution of these phages involved extensive horizontal exchange of genetic material. Filamentous phages which use type IV pili as receptors were found to belong to different branches. While one of these branches is represented by CTX Phi, which uses the toxin-coregulated pilus as its receptor, at least four evolutionarily diverged phages share a common receptor MSHA, and most of these phages mediate horizontal gene transfer. Since MSHA is present in a wide variety of V. cholerae strains and is presumed to express in the environment, diverse filamentous phages using this receptor are likely to contribute significantly to V. cholerae evolution.