Discovery of high-confidence single nucleotide polymorphisms from large-scale de novo analysis of leaf transcripts of Aegilops tauschii, a wild wheat progenitor.
Discovery of high-confidence single nucleotide polymorphisms from large-scale de novo analysis of leaf transcripts of Aegilops tauschii, a wild wheat progenitor.
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DOI：
10.1093/dnares/dss028
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Takumi S
中科院分区：
文献类型：
--
作者：
Iehisa JC;Shimizu A;Sato K;Nasuda S;Takumi S
高分辨率遗传图谱的构建对于遗传学和基因组学研究以及分子育种具有重要意义。单核苷酸多态性（SNP）是遗传变异的主要类型，可用作分子标记。节节麦是普通小麦的D基因组供体，是小麦改良的重要遗传资源。我们以前的研究表明，Ae。节节麦种质在系谱学上可分为两个主要谱系。在这项研究中，两个Ae.对来自每个谱系（谱系1（L1）和2（L2））的节节麦种质进行测序，在从头组装读数后产生9435个SNP和739个插入/缺失多态性（indels）。基于36个重叠群序列，在20个不同的Ae上验证了31个SNP和6个indel。桃木属的加入。由于几乎所有的SNP标记在L1和L2之间都是多态的，并且普通小麦的D基因组供体被假定为属于L2，因此这些标记可用于普通小麦品种与L1衍生的合成小麦之间的杂交的D基因组分型。由于小麦和大麦染色体之间存在保守的同线性，高密度表达序列标签大麦图谱和大麦中的假定基因顺序可用于开发小麦目标染色体区域的标记。
Construction of high-resolution genetic maps is important for genetic and genomic research, as well as for molecular breeding. Single nucleotide polymorphisms (SNPs) are the predominant class of genetic variation and can be used as molecular markers. Aegilops tauschii, the D-genome donor of common wheat, is considered a valuable genetic resource for wheat improvement. Our previous study implied that Ae. tauschii accessions can be genealogically divided into two major lineages. In this study, the transcriptome of two Ae. tauschii accessions from each lineage, lineage 1 (L1) and 2 (L2), was sequenced, yielding 9435 SNPs and 739 insertion/deletion polymorphisms (indels) after de novo assembly of the reads. Based on 36 contig sequences, 31 SNPs and six indels were validated on 20 diverse Ae. tauschii accessions. Because almost all of the SNP markers were polymorphic between L1 and L2, and the D-genome donor of common wheat is presumed to belong to L2, these markers are available for D-genome typing in crosses between common wheat varieties and L1-derived synthetic wheat. Due to the conserved synteny between wheat and barley chromosomes, the high-density expressed sequence tag barley map and the hypothetical gene order in barley can be applied to develop markers on target chromosomal regions in wheat.
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影响因子：
7.4
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