Highly Diverse and Seasonally Dynamic Protist Community in a Pristine Peat Bog
Highly Diverse and Seasonally Dynamic Protist Community in a Pristine Peat Bog
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DOI：
10.1016/j.protis.2010.05.003
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发表时间：
2011-01-01
期刊：
影响因子：
2.5
通讯作者：
Garcia, Purificacion Lopez
中科院分区：
文献类型：
--
作者：
Lara, Enrique;Mitchell, Edward A. D.;Garcia, Purificacion Lopez
以小亚基核糖体RNA基因(SSU RDNA)的扩增、克隆和测序为基础的非培养分子方法是研究微生物多样性的有力工具。尽管如此，包括泥炭地在内的许多生态系统的真核微生物多样性尚未得到太多关注。在一个完整的季节周期中，我们通过分子调查分析了瑞士汝拉山脉原始泥炭沼泽中心的水中真核生物的多样性。从五个不同的时间样本构建的克隆文库显示，原生生物具有高度的多样性，代表了所有主要的真核生物门。此外，四种序列类型不能被归入任何已知的高水平真核生物分类群，但在相当好的统计支持下一起分支在一起，增加了一个新的、深分支的真核生物分支的可能性。系统类型的季节模式分析表明，真核生物群落在暖期(春末和夏季)和冷期(秋和冬季)之间发生了明显的变化。金藻在寒冷时期占主导地位，而被微体阿米巴(Arcellinida和Euglphida)和其他几个类群在夏季达到顶峰。在给定的采样时间内，一些系统类型(如隐形单胞藻和perkinsid)很丰富，然后几乎消失，这表明了水华般的动态。(C)2010年，爱思唯尔股份有限公司出版。
Culture-independent molecular methods based on the amplification, cloning and sequencing of small-subunit ribosomal RNA genes (SSU rDNAs) are powerful tools to study the diversity of microorganisms. Despite so, the eukaryotic microbial diversity of many ecosystems, including peatlands has not yet received much attention. We analysed the eukaryotic diversity by molecular surveys in water from the centre of a pristine Sphagnum-dominated peatland in the Jura Mountains of Switzerland during a complete seasonal cycle. The clone libraries constructed from five different temporal samplings revealed a high diversity of protists with representatives of all major eukaryotic phyla. In addition, four sequence types could not be assigned to any known high-level eukaryotic taxon but branched together with a rather good statistic support, raising the possibility of a novel, deep branching eukaryotic clade. The analysis of seasonal patterns of phylotypes showed a clear change in the eukaryotic communities between the warm period (late spring and summer) and the cold period (autumn and winter). Chrysophytes dominated the samples in the cold period while testate amoebae (Arcellinida and Euglyphida) and a few other groups peaked in summer. A few phylotypes (such as a cryptomonad and a perkinsid) were abundant at given sampling times and then almost disappeared, suggesting bloom-like dynamics. (C) 2010 Published by Elsevier GmbH.