Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction
Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction
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骨髓源性内皮祖细胞：角膜内皮功能障碍的一种有前景的治疗替代方案
DOI：
10.1159/000319797
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发表时间：
2011-01-01
影响因子：
2.7
通讯作者：
Fan, Xianqun
中科院分区：
文献类型：
--
作者：
Shao, Chunyi;Fu, Yao;Fan, Xianqun
背景：全球供体角膜的短缺推动了生物工程角膜的发展。虽然角膜内皮细胞(CEC)和前体细胞与各种载体材料一起构建角膜内皮，但目前所有的选择都需要角膜组织，而且还受到供体角膜稀缺的限制。在此，我们探讨了诱导骨髓源性内皮祖细胞(BEPC)分化为CEC修复角膜内皮缺损的可行性。材料和方法：从人胎儿骨髓中分离出BEPC，并用多种抗原标记进行鉴定。BEPC与CEC在含CEC条件培养液的Transwell系统中共培养10天，然后进行细胞转分化实验。以猪角膜脱细胞基质(PCACM)为载体，将诱导的BEPC移植到剥脱Descemet膜和内皮的猫角膜上。结果：诱导的BEPC类似于CEC，呈多角形，表达水通道蛋白-1、紧密相对的细胞连接和神经元特异性烯醇化酶。术后28天，植入诱导BEPC的PCACM角膜逐渐恢复透明。结论：人胎儿BEPC在体外可转分化为角膜内皮样细胞。诱导的BEPC的特征表明，它们可能有助于角膜内皮功能障碍的修复。
Background: The global shortage of donor corneas has motivated the development of bioengineered corneas. Although corneal endothelium has been reconstituted using corneal endothelial cells (CEC) and precursor cells with various carrier materials, all of the current options require corneal tissue and are also limited by the scarcity of donor corneas. Here, we explored the feasibility of inducing bone marrow-derived endothelial progenitor cells (BEPC) to differentiate into CEC for the repair of corneal endothelial defects. Materials and Methods: BEPC were isolated from human fetal bone marrow, and identified using several antigen markers. BEPC were cocultured with CEC for 10 days in a transwell system with conditioned medium from CEC, and cell transdifferentiation was then examined. With a porcine corneal acellular matrix (PCACM) as the carrier, the induced BEPC were transplanted onto a cat’s cornea from which Descemet’s membrane and the endothelium had been stripped. Results: The induced BEPC resembled CEC in polygonal shape, expressing aquaporin-1, tightly opposed cell junctions, and neurone-specific enolase. Twenty-eight days after surgery, the transparency gradually returned to the corneas transplanted with the induced BEPC on PCACM. Conclusions: Human fetal BEPC transdifferentiate into corneal endothelial-like cells in vitro. Features of the induced BEPC indicated that they may be useful for the repair of corneal endothelial dysfunction.