Cartilage Tissue Engineering Using Electrospun PCL Nanofiber Meshes and MSCs
Cartilage Tissue Engineering Using Electrospun PCL Nanofiber Meshes and MSCs
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DOI：
10.1021/bm100476r
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发表时间：
2010-12-01
影响因子：
6.2
通讯作者：
Neves, N. M.
中科院分区：
文献类型：
--
作者：
Alves da Silva, M. L.;Martins, A.;Neves, N. M.
间充质干细胞（MSC）已被公认为具有分化为不同组织（如骨、软骨或脂肪组织）细胞的能力，因此对潜在的治疗策略具有极大的兴趣。从接受膝关节置换术的患者的人骨髓抽吸物中分离粘附的、集落形成的成纤维细胞，并通过流式细胞术表征MSC表型。然后，将细胞接种到电纺聚己内酯纤维网上，并在多室流动灌注生物反应器中培养，以确定其产生软骨细胞外基质的能力。结果表明，流动灌注生物反应器增加了hBM-MSCs的软骨分化，无论是通过形态学和RT-PCR分析证实。软骨相关基因如聚集蛋白聚糖、II型胶原和Sox 9表达。还通过组织学程序检测ECM沉积。样品中存在II型胶原以及I型胶原。尽管未获得基因表达的统计学显著值，但其他结果支持选择生物反应器进行此类培养。
Mesenchymal stem cells (MSCs) have been recognized for their ability to differentiate into cells of different tissues such as bone, cartilage, or adipose tissue, and therefore are of great interest for potential therapeutic strategies. Adherent, colony-forming, fibroblastic cells were isolated from human bone marrow aspirates, from patients undergoing knee arthroplasties, and the MSCs phenotype characterized by flow cytometry. Afterward, cells were seeded onto electrospun polycaprolactone nanofiber meshes and cultured in a multichamber flow perfusion bioreactor to determine their ability to produce cartilagineous extracellular matrix. Results indicate that the flow perfusion bioreactor increased the chondrogenic differentiation of hBM-MSCs, as confirmed either by morphological and RT-PCR analysis. Cartilage-related genes such as aggrecan, collagen type II, and Sox9 were expressed. ECM deposition was also detected by histological procedures. Collagen type II was present in the samples, as well as collagen type I. Despite no statistically significant values being obtained for gene expression, the other results support the choice of the bioreactor for this type of culture.