Root isoprene formation alters lateral root development.
Root isoprene formation alters lateral root development.
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DOI：
10.1111/pce.13814
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发表时间：
2020-06
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影响因子：
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通讯作者：
Maja Miloradovic van Doorn;J. Merl-Pham;A. Ghirardo;S. Fink;A. Polle;J. Schnitzler;M. Rosenkranz
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作者：
Maja Miloradovic van Doorn;J. Merl-Pham;A. Ghirardo;S. Fink;A. Polle;J. Schnitzler;M. Rosenkranz
异戊二烯是一种C5挥发性有机化合物，它可以保护植物地上组织免受非生物胁迫，如短期高温和活性氧（ROS）的积累。在这里，我们发现了异戊二烯在植物地下组织中的新作用。通过对黑杨异戊二烯合成酶（PcISPS）启动子报告植物的分析，发现PcISPS启动子在根的维管组织、分化区和根冠等部位具有活性。用生长素或盐处理根增加了PcISPS启动子在这些位点的活性，特别是在发育中的侧根（LR）中。转基因，异戊二烯非排放白杨根揭示了积累的O2 -在相同的根区域，PcISPS启动子活性本地化。异戊二烯排放的情况下，此外，增加了LRs的形成。抑制NAD（P）H氧化酶活性抑制LR的发展，表明在这个过程中的ROS的参与。细根蛋白质组的分析揭示了一个组成性转变的量的氧化还原平衡，信号和发展相关的蛋白质，如超氧化物歧化酶，各种过氧化物酶和亚油酸9 S-脂氧合酶，异戊二烯非排放白杨根。总之，我们的研究结果表明，异戊二烯的ROS相关的功能，最终共同调节植物内部的信号网络和根组织的发育过程。本文受版权保护。All rights reserved.
Isoprene is a C5 volatile organic compound, which can protect aboveground plant tissue from abiotic stress such as short-term high temperatures and accumulation of reactive oxygen species (ROS). Here, we uncover new roles for isoprene in the plant belowground tissues. By analyzing Populus x canescens isoprene synthase (PcISPS) promoter reporter plants, we discovered PcISPS promoter activity in certain regions of the roots including the vascular tissue, the differentiation zone and the root cap. Treatment of roots with auxin or salt increased PcISPS promoter activity at these sites, especially in the developing lateral roots (LR). Transgenic, isoprene non-emitting poplar roots revealed an accumulation of O2 - in the same root regions where PcISPS promoter activity was localized. Absence of isoprene emission, moreover, increased the formation of LRs. Inhibition of NAD(P)H oxidase activity suppressed LR development, suggesting the involvement of ROS in this process. The analysis of the fine root proteome revealed a constitutive shift in the amount of several redox balance, signaling and development related proteins, such as superoxide dismutase, various peroxidases and linoleate 9S-lipoxygenase, in isoprene non-emitting poplar roots. Together our results indicate for isoprene a ROS-related function, eventually co-regulating the plant-internal signaling network and development processes in root tissue. This article is protected by copyright. All rights reserved.