BASEMENT-MEMBRANE COMPLEXES WITH BIOLOGICAL-ACTIVITY
BASEMENT-MEMBRANE COMPLEXES WITH BIOLOGICAL-ACTIVITY
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DOI：
10.1021/bi00350a005
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发表时间：
1986-01-28
期刊：
影响因子：
2.9
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
--
作者：
KLEINMAN, HK;MCGARVEY, ML;MARTIN, GR
我们研究了从EHS肿瘤基底膜制备的提取物中基底膜分子的重建。在生理条件下，在加入IV型胶原和硫酸乙酰肝素蛋白聚糖的情况下，形成凝胶样结构，其超微结构表现为相互连接的薄片，类似于基底膜的薄层致密区。重组结构的主要成分包括层粘连蛋白、IV型胶原、硫酸乙酰肝素蛋白聚糖、巢蛋白和巢蛋白。这些组分在重构时以恒定的比例混合，表明它们以确定的比例相互作用。分子筛的可溶性提取物的研究表明，层粘连蛋白，entactin，巢蛋白相关的大，但可解离的复合物，这可能是一个必要的中间体在基底膜的沉积。重组基质具有生物活性，并刺激某些细胞的生长和分化。
We have studied the reconstitution of basement membrane molecules from extracts prepared from the basement membrane of the EHS tumor. Under physiological conditions and in the presence of added type IV collagen and heparn sulfate proteglycan, gellike structures form whose ultrastructure appears as interconnected thin sheets resembling the lamina dense zone of basement membrane. The major components of the reconstituted structures include laminin, type IV collagen, heparan sulfate proteoglycan, entactin, and nidogen. These components polymerize in constant proportions on reconstitution, suggesting that they interact in defined proportions. Molecular sieve studies on the soluble extract demonstrate that laminin, entactin, and nidogen are associated in large but dissociable complexes which may be a necessary intermediate in the deposition of basement membrane. The reconstituted matrix was biologically active and stimulated the growth and differentiation of certain cells.