Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.
Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.
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DOI：
10.1084/jem.136.2.207
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发表时间：
1972-08-01
期刊：
影响因子：
--
通讯作者：
Wigzell H
中科院分区：
文献类型：
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作者：
Jondal M;Holm G;Wigzell H
用（a）细胞表面高密度免疫球蛋白决定簇和（B）细胞表面存在C ′ 3受体这两个标准来定义骨髓来源的淋巴细胞，间接表明所有或至少大部分人胸腺来源的淋巴细胞在一定条件下将与绵羊红细胞（SRBC）形成非免疫反应。几乎所有来自两个不同供体的胸腺细胞都形成了红细胞。SRBC受体不是通过免疫球蛋白受体形成的，仅在活细胞周围形成。由于EDTA会阻断玫瑰花结的形成，因此玫瑰花结的形成需要正二价离子。碘乙酸钠也会阻断玫瑰花结的形成，证明对完整糖酵解途径的依赖性。玫瑰花结的形成取决于温度，在37°C时不会出现。用胰蛋白酶处理淋巴细胞将消除它们的SRBC结合能力，这种能力不能通过用新鲜供体血清或胎牛血清处理它们而恢复，但在培养淋巴细胞后会重新出现。这表明，这些roximine是由活细胞表面上的一种快速释放或代谢的受体物质形成的，其表现为细胞本身产生的胰蛋白酶敏感结构。
By using the two criteria (a) high density of immunoglobulin determinants on the cell surface and (b) presence of receptors for C'3 on the cell surface for defining bone marrow-derived lymphocytes, it is indirectly shown that all or at least a major population of human thymus-derived lymphocytes under certain conditions will form nonimmune rosettes with sheep red blood cells (SRBC). Almost all thymocytes tested from two different donors formed rosettes. The SRBC rosettes are not formed by virtue of immunoglobulin receptors and form only around living cells. Positive bivalent ions are required for rosette formation since EDTA will block rosette formation. Sodium iodoacetate will also block rosette formation demonstrating the dependence on an intact glycolytic pathway. Rosette formation is temperature dependent and will not appear at 37°C. Trypsin treatment of lymphocytes will abolish their SRBC-binding ability which cannot be restored by treating them with fresh donor serum or fetal calf serum, but which will reappear after culturing the lymphocytes. It is suggested that these rosettes are formed by a rapidly released or metabolized receptor substance on the living cell surface which behaves as a trypsin-sensitive structure produced by the cells themselves.