Demonstration of a RNA-dependent nuclear interaction between the promyelocytic leukaemia protein and glyceraldehyde-3-phosphate dehydrogenase
Demonstration of a RNA-dependent nuclear interaction between the promyelocytic leukaemia protein and glyceraldehyde-3-phosphate dehydrogenase
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DOI：
10.1042/bj3350691
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发表时间：
1998-11-01
影响因子：
4.1
通讯作者：
Borden, KLB
中科院分区：
文献类型：
--
作者：
Carlile, GW;Tatton, WG;Borden, KLB
早幼粒细胞白血病（蛋白质）（PML）定位于称为 PML 核体的多蛋白质复合物。我们发现 3-磷酸甘油醛脱氢酶 (GAPDH) 与 PML 发生免疫共沉淀，并与 PML 共定位于核体中。 RNase 处理破坏了 PML 和 GAPDH 共定位和共免疫沉淀的能力，表明 PML 和 GAPDH 之间的关联取决于 RNA 的存在。 PML 体的破坏有助于减少急性早幼粒细胞白血病的细胞凋亡，而 GAPDH 会诱导细胞凋亡的神经元死亡。 GAPDH-PML 相互作用可能参与细胞凋亡的调节。
The promyelocytic leukaemia (protein) (PML) localizes to multi-protein complexes known as PML nuclear bodies. We found that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) coimmunoprecipitates with PML and col-localizes with PML in nuclear bodies. RNase treatment disrupts the ability of PML and GAPDH to both co-localize and co-immunoprecipitate, indicating that the association between PML an GAPDH depends on the presence of RNA. Disruption of PML bodies contributes towards reduced apoptosis in acute promyelocytic leukaemia and GAPDH induces apoptotic neuronal death. The GAPDH-PML interaction may be involved in the regulation of apoptosis.