RelB is essential for the development of myeloid-related CD8α- dendritic cells but not of lymphoid-related CD8α+ dendritic cells
RelB is essential for the development of myeloid-related CD8α- dendritic cells but not of lymphoid-related CD8α+ dendritic cells
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DOI：
10.1016/s1074-7613(00)80649-4
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发表时间：
1998-12-01
期刊：
影响因子：
32.4
通讯作者：
Shortman, K
中科院分区：
文献类型：
--
作者：
Wu, L;D'Amico, A;Shortman, K
转录因子RelB在树突状细胞（DC）发育中起重要作用，但涉及DC的类型尚不清楚。在这里，我们报道了RelB mRNA在CD8 α (-) DEC-205(-) DC中强烈表达，而在CD8 α (+) DEC-205(+) DC中仅弱表达。此外，CD8 α (+) DEC-205(+) DC在RelB缺失小鼠中存在并具有功能，DC缺陷主要存在于CD8 α (-) DEC-205（-）人群中。通过构建骨髓嵌合小鼠，我们证明了RelB无胸腺DC的部分缺陷是胸腺结构破坏的继发效应。然而，脾CD8 α (-) DEC-205(-) DC的缺乏是RelB突变对干细胞的直接内在影响。因此，RelB选择性地调控髓系相关的DC谱系。
The transcription factor RelB had been shown to be important for dendritic cell (DC) development, but the type of DC involved was not clear. Here, we report that RelB mRNA is expressed strongly in CD8 alpha(-) DEC-205(-) DC but only weakly in CD8 alpha(+) DEC-205(+) DC. In addition, CD8 alpha(+) DEC-205(+) DC are present and functional in RelB null mice, the DC deficiency being mainly in the CD8 alpha(-) DEC-205(-) population. By constructing bone-marrow chimeric mice, we demonstrate that the partial deficiency in RelB null thymic DC is a secondary effect of disrupted thymic architecture. However, the deficiency in splenic CD8 alpha(-) DEC-205(-) DC is a direct, stem cell intrinsic effect of the RelB mutation. Thus, RelB selectively regulates a myeloid-related DC lineage.