Attoliter-scale dispensing in nanofluidic channels
Attoliter-scale dispensing in nanofluidic channels
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DOI：
10.1021/ac061814m
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发表时间：
2007-02-15
影响因子：
7.4
通讯作者：
Jacobson, Stephen C.
中科院分区：
文献类型：
--
作者：
Kovarik, Michelle L.;Jacobson, Stephen C.
随着制造技术的改进，具有纳米尺度尺寸的功能性流体装置正在迅速开发用于化学分析。在这里，我们提出了在纳米通道中的流体分配，注射体积范围从42 aL到4.1 fL。混合聚（二甲基硅氧烷）和玻璃纳米通道，130 nm深和580 nm宽或130 nm深和670 nm宽的设备，被用来评估两个样品分配方案，修改捏和门控注射。通过直接从模拟输出板施加高达10 V的电压而不放大，在纳米通道中产生适度的电场强度（0.2-2 kV/cm）并能够快速分配和分析（10-100 ms）来实现动电传输。
As fabrication techniques improve, functional fluidic devices with nanometer scale dimensions are rapidly being developed for chemical analysis. Here, we present fluid dispensing in nanochannels with injection volumes ranging from 42 aL to 4.1 fL. Devices with hybrid poly(dimethylsiloxane) and glass nanochannels, 130 nm deep and 580 nm wide or 130 nm deep and 670 nm wide, were used to evaluate two sample dispensing schemes, modified pinched and gated injections. Electrokinetic transport was achieved by applying up to 10 V directly from an analog output board without amplification, producing modest electric field strengths in the nanochannels (0.2-2 kV/cm) and enabling rapid dispensing and analysis (10-100 ms).