Structural proteins of two salmonid rhabdoviruses
Structural proteins of two salmonid rhabdoviruses
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两种鲑鱼弹状病毒的结构蛋白
DOI：
10.1128/jvi.15.4.733-738.1975
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发表时间：
1975
影响因子：
5.4
通讯作者：
R. Wagner
中科院分区：
文献类型：
--
作者：
P. McAllister;R. Wagner
纯化的传染性造血细胞坏死（IHN）病毒和出血性败血症（VHS）病毒（埃格特维德病毒）各含有5种结构蛋白，分别命名为L、G、N、M-1和M-2。IHN病毒多肽的分子量估计分别为157，000、72，000、40，000、25，000和20，000，而VHS病毒多肽的分子量估计分别为157，000、74，000、41，000、21，500和19，000。通过证明[3 H]葡糖胺选择性掺入两种病毒的指定G蛋白，证实了糖蛋白（G）的碳水化合物组成。通过将[32 P]正磷酸盐掺入IHN病毒的N和M-1蛋白以及VHS病毒的N蛋白中来鉴定磷蛋白。每种病毒的糖蛋白通过在低盐缓冲液中用Triton X-100处理而选择性地溶解，而M-1和M-2蛋白沿着G蛋白通过在0.43 M NaCl中用Triton X-100溶解。鲑科弹状病毒的蛋白质组成与狂犬病病毒组的蛋白质组成比水疱性口炎病毒组更接近。
Purified infectious hematopoietic necrosis (IHN) virus and the virus of haemorrhagic septicaemia (VHS) (Egtved virus) each contain five structural proteins which were designated L, G, N, M-1, and M-2. The IHN viral polypeptides have molecular weights estimated to be 157,000, 72,000, 40,000, 25,000 and 20,000, respectively, whereas those of VHS viral polypeptides are estimated to be 157,000 74,000, 41,000, 21,500, and 19,000, respectively. The carbohydrate composition of the glycoprotein (G) was confirmed by demonstrating selective incorporation of [3H]glucosamine into the designated G protein of both viruses. Phosphoproteins were identified by incorporation of [32P]orthophosphate into the N and M-1 proteins of IHN virus and into the N protein of VHS virus. The glycoprotein of each virus was selectively solubilized by treatment with Triton X-100 in low salt buffer, whereas the M-1, and M-2 proteins along with the G protein were solubilized by Ttition X-100 in 0.43 M NaCl. The protein composition of the salmonid rhabdoviruses resembles that of the rabies virus group more closely than the vesicular stomatitis virus group.