Mass determination by inelastic electron scattering in an energy-filtering transmission electron microscope with slow-scan CCD camera
Mass determination by inelastic electron scattering in an energy-filtering transmission electron microscope with slow-scan CCD camera
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DOI：
10.1006/jsbi.1997.3861
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发表时间：
1997-06-01
影响因子：
3
通讯作者：
Aebi, U
中科院分区：
文献类型：
--
作者：
Feja, B;Durrenberger, M;Aebi, U
一种确定生物分子组件质量的方法利用了薄样品质量与入射电子散射分数之间的线性关系，电子散射质量测定首先通过扫描透射电子显微镜（STEM）进行，通过环形暗场探测器收集弹性散射电子，在能量过滤透射电子显微镜（EFTEM）中，由非弹性散射电子形成的暗保持图像由高度线性和敏感的慢扫描CCD相机记录，将收集到的散射电子的作用与样品质量相关的校准因子可以通过使用已知分子质量的粒子作为参考标准来确定，处理由慢扫描CCD相机获得的数字暗保持图像可以得到细颗粒的质量，每长度的长丝的质量，为了验证这一方法，我们在EFTEM中对一些典型的生物样品进行了评估，并将所得结果与STEM的结果进行了比较。数据表明，配备高性能慢扫描CCD相机的EFTEM是一种有效的电子光学设备，用于测定生物分子组件的质量，其精度和再现性与STEM相当。(C) 1997学术出版社。
One method of determining the mass of biomolecular assemblies takes advantage of the Linear relationship between the mass of a thin sample and the scattered fraction of the incident electrons, Mass determination by electron scattering was first performed by scanning transmission electron microscopy (STEM), collecting the elastically scattered electrons by an annular dark-field detector, In the energy-filtering transmission electron microscope (EFTEM), the dark-held images formed by inelastically scattered electrons are recorded with a highly Linear and sensitive slow-scan CCD camera, A calibration factor relating the collected fi action of scattered electrons to the mass of a sample can be determined by using a particle of known molecular mass as a reference standard, Processing of the digital dark-held images obtained by the slow-scan CCD camera allows the mass of thin particles, the mass per length of filaments, or the mass per area of planar samples to be determined, To validate this approach, a number of typical biological samples were evaluated in the EFTEM and the results obtained were compared with those from STEM, The data presented demonstrate that an EFTEM equipped with a high-performance slow-scan CCD camera is an effective electron optical device for mass determination of biomolecular assemblies with an accuracy and reproducibility comparable to that achieved by STEM. (C) 1997 Academic Press.