Micro-RNA profiling in kidney and bladder cancers
Micro-RNA profiling in kidney and bladder cancers
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DOI：
10.1016/j.urolonc.2007.01.019
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发表时间：
2007-09-01
影响因子：
2.7
通讯作者：
Baffa, Raffaele
中科院分区：
文献类型：
--
作者：
Gottardo, Fedra;Liu, Chang Gong;Baffa, Raffaele
目的：微rna是一类具有基因表达调节活性的小分子非编码rna。最近，微rna基因被发现在几种类型的癌症中异常表达。为了研究微rna在人肾癌和膀胱癌中的作用，我们分析了245个微rna在肾和膀胱原发肿瘤中的表达谱。方法与材料：共纳入27例肾脏标本（癌20例，良性肾肿瘤4例，正常实质3例）和27例膀胱标本（尿路上皮癌25例，正常粘膜2例）。总RNA在我们实验室开发的用于微RNA分析的寡核苷酸微芯片上进行杂交。该芯片包含368个探针，一式三份，对应245个人和小鼠的微rna基因。结果：在肾癌组织中，miR-28、miR-185、miR-27、let-7f-2等4组人微rna表达水平明显高于正常肾组织（P < 0.05）。与正常膀胱粘膜相比，人微rna miR-223、miR-26b、miR-221、miR-103-1、miR-185、miR-23b、miR-203、miR-17-5p、miR-23a和miR-205在膀胱癌中显著上调（P < 0.05）。在研究的肾癌中，微rna的表达在不同分期之间没有差异，而在膀胱癌中，随着肿瘤-淋巴结-转移分期的增加，miR-26b呈中度下降趋势（P = 0.082）。结论：我们的研究结果表明，不同的微rna在肾癌和膀胱癌中被解除调控，表明这些基因参与了这些恶性肿瘤的发生和进展。需要进一步的研究来阐明微rna在肿瘤转化中的作用，并测试微rna微阵列作为诊断和预后工具的潜在临床用途。(C) 2007爱思唯尔公司版权所有。
Objectives: Micro-RNAs are a group of small noncoding RNAs with modulator activity of gene expression. Recently, micro-RNA genes were found abnormally expressed in several types of cancers. To study the role of the micro-RNAs in human kidney and bladder cancer, we analyzed the expression profile of 245 micro-RNAs in kidney and bladder primary tumors.Methods and materials: A total of 27 kidney specimens (20 carcinomas, 4 benign renal tumors, and 3 normal parenchyma) and 27 bladder specimens (25 urothelial carcinomas and 2 normal mucosa) were included in the study. Total RNA was used for hybridization on an oligonucleotide microchip for micro-RNA profiling developed in our laboratories. This microchip contains 368 probes in triplicate, corresponding to 245 human and mouse micro-RNA genes.Results: A set of 4 human micro-RNAs (miR-28, miR-185, miR-27, and let-7f-2) were found significantly up-regulated in renal cell carcinoma (P < 0.05) compared to normal kidney. Human micro-RNAs miR-223, miR-26b, miR-221, miR-103-1, miR-185, miR-23b, miR-203, miR-17-5p, miR-23a, and miR-205 were significantly up-regulated in bladder cancers (P < 0.05) compared to normal bladder mucosa. Of the kidney cancers studied, there was no differential micro-RNA expression across various stages, whereas with increasing tumor-nodes-metastasis staging in bladder cancer, miR-26b showed a moderate decreasing trend (P = 0.082).Conclusions: Our results show that different micro-RNAs are deregulated in kidney and bladder cancer, suggesting the involvement of these genes in the development and progression of these malignancies. Further studies are needed to clarify the role of micro-RNAs in neoplastic transformation and to test the potential clinical usefulness of micro-RNAs microarrays as diagnostic and prognostic tool. (C) 2007 Elsevier Inc. All rights reserved.