Activation of mu opioid receptors in the ventrolateral periaqueductal gray inhibits reflex micturition in anesthetized rats
Activation of mu opioid receptors in the ventrolateral periaqueductal gray inhibits reflex micturition in anesthetized rats
复制标题
DOI：
10.1016/j.neulet.2004.03.055
复制
发表时间：
2004-06-10
影响因子：
2.5
通讯作者：
Millington, WR
中科院分区：
文献类型：
--
作者：
Matsumoto, S;Levendusky, MC;Millington, WR
本研究验证了吗啡和其他阿片类药物通过激活中脑导水管周围灰质（PAG）区域的μ阿片受体引起尿潴留的假设。将选择性mu、delta和kappa受体激动剂微量注射到麻醉大鼠的PAG中，并在连续盐水输注期间记录膀胱收缩的幅度和发生率。通过股动脉导管监测动脉压。将μ受体激动剂DAMGO微量注射到腹外侧PAG（vlPAG）中可完全抑制容量诱发的膀胱收缩。膀胱收缩在DAMGO注射后5分钟内停止，并且在20分钟记录期的剩余时间内基本上检测不到。将δ受体激动剂DPDPE微量注射到vlPAG中并不显著影响膀胱收缩的幅度或分离收缩的时间间隔。κ受体激动剂U-69593没有引起明显的幅度变化，但增加膀胱收缩之间的间隔显着。将DAMGO、DPDPE或U-69593微量注射到PAG的外侧或背外侧柱中无效。将DAMGO注射到vlPAG中增加动脉压，而DPDPE和U-69593产生小但显著的降压反应。这些数据支持vlPAG中的mu和kappa受体参与排尿反射的假设。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
This study tested the hypothesis that morphine and other opiates cause urinary retention by activating mu opioid receptors in the midbrain periaqueductal gray (PAG) region. Selective mu, delta and kappa receptor agonists were microinjected into the PAG of urethane-anesthetized rats and the amplitude and incidence of bladder contractions were recorded during continuous saline infusion. Arterial pressure was monitored through a femoral artery catheter. Microinjection of the mu receptor agonist DAMGO into the ventrolateral PAG (vlPAG) suppressed volume-evoked bladder contractions completely. Bladder contractions ceased within 5 min of DAMGO injection and remained essentially undetectable for the rest of the 20 min recording period. Microinjection of the delta receptor agonist DPDPE into the vlPAG did not significantly affect either the amplitude of bladder contractions or the time interval separating contractions. The kappa receptor agonist U-69593 caused no discernible change in amplitude but increased the interval between bladder contractions significantly. Microinjection of DAMGO, DPDPE or U-69593 into the lateral or dorsolateral PAG columns was ineffective. DAMGO injection into the vlPAG increased arterial pressure whereas DPDPE and U-69593 produced a small but significant depressor response. These data support the hypothesis that mu and kappa receptors in the vlPAG participate in the micturition reflex. (C) 2004 Elsevier Ireland Ltd. All rights reserved.