Bacillus camelliae sp nov., isolated from Pu'er tea
Bacillus camelliae sp nov., isolated from Pu'er tea
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从普洱茶中分离出的山茶芽孢杆菌新种
DOI：
10.1099/ijsem.0.002542
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发表时间：
2018-02-01
影响因子：
2.8
通讯作者：
Li, Wenying
中科院分区：
文献类型：
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作者：
Niu, Lili;Xiong, Mengjie;Li, Wenying
从普洱熟茶中分离到一株革兰氏阳性产孢杆状好氧菌7578-1（T）。基于16 S rRNA基因序列相似性比较，菌株7578-1（T）被归为芽孢杆菌属，并且似乎与模式菌株Bacillusshackletonii LMG 18435（T）密切相关。（98.4%）、嗜酸芽孢杆菌DSM 14745（T）（97.6%）、地衣芽孢杆菌KACC 18426（T）（97.2%）和腐殖芽孢杆菌KCTC 13944（T）（96.7%）。菌株7578 - 1（T）的脂肪酸组成以异碳15：0、反异碳15：0和反异碳17：0为主，支持菌株7578-1（T）属于芽孢杆菌属。该菌株具有细胞壁型A1 γ肽聚糖，其中内消旋二氨基庚二酸作为诊断二氨基酸。主要的甲基萘醌是MK-7（95%）。主要极性脂质为二磷脂酰甘油、磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、一种未鉴别磷脂和一种未鉴别脂质。经OrthoANIu分析，7578-1（T）株与其最接近种的核苷酸同源性为67.8-82.4%。菌株7578-1（T）与近缘种的模式菌株之间的DNA-DNA相关性值为17- 39%，再次表明菌株7578-1（T）代表芽孢杆菌属中的一个新种。菌株7578-1（T）的DNA G+C含量为36.0mol%。根据所提出的多相证据，菌株7578-1（T）被认为是芽孢杆菌属的一个新种，我们建议将其命名为芽孢杆菌Camellia sp. nov.。模式菌株为7578-1（T）（=CGMCC 1.15374（T）=KCTC 33845（T））。
A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1(T), was isolated from ripened Pu'er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1(T) was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletonii LMG 18435(T) (98.4 %), Bacillus acidicola DSM 14745(T) (97.6 %), Bacillus paralicheniformis KACC 18426(T) (97.2 %) and Bacillus ginsengihumi KCTC 13944(T) (96.7 %). The fatty acid profile containing the major fatty acids, iso-C-15 : 0, anteiso-C-15 : 0 and anteiso-C-17 : 0 supported the allocation of strain 7578-1(T) to the genus Bacillus. The strain had a cell-wall type A1 gamma peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-7 (95 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unidentified phospholipid and one unidentified lipid. The average nucleotide identity values between strain 7578-1(T) and its most closely related species were 67.8-82.4% by OrthoANIu analysis. The DNA-DNA relatedness value between strain 7578-1(T) and the type strains of closely related species were 17-39 %, again indicating that strain 7578-1(T) represented a novel species in the genus Bacillus. The DNA G+C content of strain 7578-1(T) was 36.0 mol%. On the basis of the presented polyphasic evidence, strain 7578-1(T) is considered to represent a novel species of the genus Bacillus, for which we propose the name Bacillus camelliae sp. nov. The type strain is 7578-1(T) (=CGMCC 1.15374(T) =KCTC 33845(T)).