EVOLUTIONARY VARIATION OF THE CCAAT-BINDING TRANSCRIPTION FACTOR NF-Y
EVOLUTIONARY VARIATION OF THE CCAAT-BINDING TRANSCRIPTION FACTOR NF-Y
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DOI：
10.1093/nar/20.5.1087
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发表时间：
1992-03-11
影响因子：
14.9
通讯作者：
MATHIS, D
中科院分区：
文献类型：
--
作者：
LI, XY;MANTOVANI, R;MATHIS, D
NF-Y 是一种 CCAAT 特异性转录因子，被认为参与多种真核基因的调节。 它显示出与酵母因子 HAP2/3 惊人的序列相似性。 为了追溯其进化历史，我们成功地从植物和几种脊椎动物中分离出 NF-Y cDNA 克隆。 序列保守模式描绘了潜在的功能域：一个高度保守的中心域，可能负责 DNA 结合和亚基相互作用；进化上更灵活的侧翼区域，其中变异性聚集在一起，使可能参与蛋白质-蛋白质接触的保守谷氨酰胺或酸性氨基酸个体化。
NF-Y is a CCAAT-specific transcription factor thought to be involved in the regulation of a variety of eukaryotic genes. It shows a striking sequence similarity with the yeast factor HAP2/3. In an attempt to trace back its evolutionary history, we succeeded in isolating NF-Y cDNA clones from a plant and from several species of vertebrates. The patterns of sequence conservation delineate potential functional domains: A central, highly conserved, domain likely responsible for DNA-binding and subunit interaction; more evolutionarily flexible flanking regions, in which variability is clustered, individualizing conserved glutamine or acidic amino-acids putatively involved in protein-protein contacts.