Decolorization of rhodamine B and congo red by partial purified laccase from Lentinus polychrous Lév
Decolorization of rhodamine B and congo red by partial purified laccase from Lentinus polychrous Lév
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DOI：
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发表时间：
2010-12
影响因子：
0.5
通讯作者：
P. Suwannawong;S. Khammuang;R. Sarnthima
中科院分区：
文献类型：
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作者：
P. Suwannawong;S. Khammuang;R. Sarnthima
研究了一种部分纯化的多色香菇漆酶对罗丹明B和刚果红的脱色作用。在存在或不存在作为氧化还原介体的2，2-连氮基双（3-乙基苯并噻唑啉-6-磺酸酯）二铵盐（ABTS）的情况下。脱色研究表明，罗丹明B在52 h内可脱色90%左右，而刚果红在3 h内可脱色75%以上。氧化还原介体的最有效浓度为0.10 mM ABTS。罗丹明B脱色的最适pH为4.0 - 5.0，最适温度为50 ℃。刚果红脱色的最适pH值为6.0-7.0，升高温度对脱色的影响不大。结果表明，该菌漆酶活性较高。多色列弗具有很高的处理废染料的潜力。
This study reports decolorization of Rhodamine B and Congo red by a partially purified laccase from Lentinus polychrous Lev . in the presence or absence of 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) diammonium salt (ABTS) as a redox mediator. The decolorization study showed that about 90% of Rhodamine B could be decolorized within 52 h, whereas Congo red could be decolorized more than 75% within 3 h. The most effective concentration of the redox mediator was 0.10 mM ABTS. The optimum pH for Rhodamine B decolorization was at pH 4.0-5.0 and optimum temperature was at 50 oC. The optimum pH for Congo red decolorization was at pH 6.0-7.0, but increasing temperature had less of an effect. The results and availability of the fungus indicated that laccase from L. polychrous Lev. has high potential for the treatment of waste dyes.