Survey of forkhead domain encoding genes in the Drosophila genome:: Classification and embryonic expression patterns
Survey of forkhead domain encoding genes in the Drosophila genome:: Classification and embryonic expression patterns
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DOI：
10.1002/dvdy.10443
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发表时间：
2004-02-01
影响因子：
2.5
通讯作者：
Frasch, M
中科院分区：
文献类型：
--
作者：
Lee, HH;Frasch, M
在果蝇中的遗传方法导致了Forkhead的鉴定，Forkhead是叉头结构域转录因子的原型，现在已知组成了一个进化上保守的蛋白质家族，在发育和分化中起着至关重要的作用。对最近发表的黑腹果蝇基因组支架序列的序列分析使我们能够确定该物种中可能完整的叉头域编码基因。在这里，我们发现果蝇基因组包含17个叉头域编码基因；其中13个基因在脊索动物物种中有直系同源物，它们的产物可以归类到已知的17个脊索动物叉头域亚类中的10个亚类。一个果蝇叉头区基因只有一个秀丽线虫的同源基因，可能代表了脊索动物中不存在的一个亚类，而其余三个基因无法分类。我们给出了该基因家族中7个以前未鉴定的成员的mRNA表达模式，并表明它们在所有三个生殖层的组织中都有表达，包括中枢和外周神经系统、表皮、唾液腺原基、内胚层、体细胞中胚层和血细胞前体细胞。此外，其中两个基因fd19B和fd102C的表达模式表明，它们在早期胚胎头部分割中作为GAP基因发挥作用。
Genetic approaches in Drosophila led to the identification of Forkhead, the prototype of forkhead domain transcription factors that are now known to comprise an evolutionarily conserved family of proteins with essential roles in development and differentiation. Sequence analysis of the recently published genomic scaffold sequence from Drosophila melanogaster has allowed us to determine the presumably full complement of forkhead domain encoding genes in this species. We show herein that the Drosophila genome contains 17 forkhead domain encoding genes; 13 of these genes have orthologs in chordate species, and their products can be assigned to 10 of the 17 forkhead domain subclasses known from chordates. One Drosophila forkhead domain gene only has a Caenorhabditis elegans ortholog and may represent a subclass that is absent in chordates, while the remaining three cannot be classified. We present the mRNA expression patterns of seven previously uncharacterized members of this gene family and show that they are expressed in tissues from all three germ layers, including central and peripheral nervous system, epidermis, salivary gland primordia, endoderm, somatic mesoderm, and hemocyte progenitors. Furthermore, the expression patterns of two of these genes, fd19B and fd102C, suggest a role for them as gap genes during early embryonic head segmentation.