The EthoVision video tracking system - A tool for behavioral phenotyping of transgenic mice
The EthoVision video tracking system - A tool for behavioral phenotyping of transgenic mice
复制标题
DOI：
10.1016/s0031-9384(01)00530-3
复制
发表时间：
2001-08-01
影响因子：
2.9
通讯作者：
Noldus, LPJJ
中科院分区：
文献类型：
--
作者：
Spink, AJ;Tegelenbosch, RAJ;Noldus, LPJJ
视频跟踪系统能够以可靠和一致的方式研究行为，并且比手动记录更长的时间段。该系统接收模拟视频信号，将每一帧数字化，并分析所得像素以确定所跟踪动物的位置（以及其他数据）。对一系列帧进行计算，以获得动物运动的一组定量描述符。EthoVision（来自Noldus Information Technology）是这种系统的具体示例，并且描述了其与转基因小鼠研究特别相关的功能。还概述了使用动物运动轨迹跟踪系统的关键实践方面，包括有关照明、标记动物、竞技场大小和采样率的提示。四个案例研究，说明了系统的各个方面：（1）禁用Munc 18-1基因的影响，清楚地显示了使用多长时间的小鼠进入一个区域在一个开放的领域的直接测量。(2)通过测量在开放场地的内部和外部区域花费的时间来量化短和长攻击潜伏期小鼠品系之间的探索行为的差异。(3)具有亚纯型CREB等位基因的小鼠在水迷宫中表现不佳，但这只有在从它们的轨迹计算一系列不同变量时才能清楚。(4)在前脑中敲除trkB受体的小鼠在水迷宫中的表现也很差，从检查轨迹图中可以立即看出，这是由于趋触性。简要讨论了视频跟踪系统的一些最新技术发展和未来可能的发展方向。(C)2001 Elsevier Science Inc. All rights reserved.
Video tracking systems enable behavior to be studied in a reliable and consistent way, and over longer time periods than if they are manually recorded. The system takes an analog video signal, digitizes each frame, and analyses the resultant pixels to determine the location of the tracked animals (as well as other data). Calculations are performed on a series of frames to derive a set of quantitative descriptors of the animal's movement. EthoVision (from Noldus Information Technology) is a specific example of such a system, and its functionality that is particularly relevant to transgenic mice studies is described. Key practical aspects of using the EthoVision system are also outlined, including tips about lighting, marking animals, the arena size, and sample rate. Four case studies are presented, illustrating various aspects of the system: (1) The effects of disabling the Munc 18-1 gene were clearly shown using the straightforward measure of how long the mice took to enter a zone in an open field. (2) Differences in exploratory behavior between short and long attack latency mice strains were quantified by measuring the time spent in inner and outer zones of an open field. (3) Mice with hypomorphic CREB alleles were shown to perform less well in a water maze, but this was only clear when a range of different variables were calculated from their tracks. (4) Mice with the trkB receptor knocked out in the forebrain also performed poorly in a water maze, and it was immediately apparent from examining plots of the tracks that this was due to thigmotaxis. Some of the latest technological developments and possible future directions for video tracking systems are briefly discussed. (C) 2001 Elsevier Science Inc. All rights reserved.