Lcg is a light-inducible and clock-controlled gene expressed in the chicken pineal gland
Lcg is a light-inducible and clock-controlled gene expressed in the chicken pineal gland
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DOI：
10.1111/j.1471-4159.2006.03712.x
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发表时间：
2006-03-01
影响因子：
4.7
通讯作者：
Fukada, Y
中科院分区：
文献类型：
--
作者：
Hatori, M;Okano, T;Fukada, Y
昼夜节律钟是一种自主的生物钟，通过接收时间线索（如光）来适应环境的24小时周期。通常，在主观夜晚的早期和晚期给出的光分别延迟和提前昼夜节律振荡器的相位。我们以前寻找鸡松果体基因，光诱导的相位依赖的方式。本研究进行了cDNA克隆和表征的基因，其表达显着上调光在主观的深夜。该基因的mRNA水平在松果体中表现出强烈的昼夜变化，在光暗周期和恒定黑暗条件下，在早期（主观）天中具有峰值，因此将其命名为Lcg（Light-inducible and Clock-controlled Gene）。鸡Lcg编码由560个氨基酸残基组成的卷曲螺旋蛋白。在鸡组织中，松果体和视网膜表现出相对较高的表达水平的LCG。LCG与γ-微管蛋白（一种中心体蛋白）在COS 7细胞中表达时共定位，LCG是第一个在中心体积累的时钟相关分子的例子。LCG与γ-微管蛋白在鸡松果体裂解物的免疫共沉淀表明昼夜节律振荡器和中心体功能之间的联系。
The circadian clock is an autonomous biological clock that is entrainable to environmental 24-h cycles by receiving time cues such as light. Generally, light given at early and late subjective night, respectively, delays and advances the phase of the circadian oscillator. We previously searched for the chicken pineal genes that are induced by light in a phase-dependent manner. The present study undertook cDNA cloning and characterization of a gene whose expression was remarkably up-regulated by light at late subjective night. The mRNA level of this gene exhibited robust diurnal change in the pineal gland, with a peak in the early (subjective) day under light-dark cycles and constant dark condition, and hence it was designated Lcg (Light-inducible and Clock-controlled Gene). Chicken Lcg encodes a coiled-coil protein composed of 560 amino acid residues. Among chicken tissues, the pineal gland and the retina exhibited relatively high expression levels of LCG. LCG was colocalized with gamma-tubulin, a centrosomal protein, when expressed in COS7 cells, and LCG is the first example of a clock-related molecule being accumulated at the centrosome. Coimmunoprecipitation of LCG with gamma-tubulin in the chicken pineal lysate suggests a link between the circadian oscillator and the centrosomal function.