Glycoproteins containing peanut-agglutinin receptors from human-peripheral-blood T-lymphocyte plasma membranes.
Glycoproteins containing peanut-agglutinin receptors from human-peripheral-blood T-lymphocyte plasma membranes.
复制标题
含有来自人外周血 T 淋巴细胞质膜的花生凝集素受体的糖蛋白。
DOI：
10.1111/j.1432-1033.1981.tb06454.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
G. Uhlenbruck
中科院分区：
文献类型：
--
作者：
G. Farrar;G. Holz;G. Uhlenbruck
具有花生凝集素受体的糖蛋白组分已从用亲和基质处理的人外周血T淋巴细胞质膜的去污剂提取物中分离，所述亲和基质包含固定在Sepharose 4 B上的花生凝集素共沉淀物。该馏分可以特异性地从亲和柱上洗脱，使用补充有0.2 M D-半乳糖的缓冲溶液，并显示，通过聚丙烯酰胺凝胶电泳在十二烷基硫酸钠（0.1%）的存在下，含有四个主要的糖蛋白组分的表观分子量为200000，190000，110000和95000。据建议，从观察到的反应性，这些糖蛋白与各种凝集素在双扩散实验中，它们具有O-糖苷和N-糖苷连接的碳水化合物。
A glycoprotein fraction possessing peanut agglutinin receptors has been isolated from detergent extracts of neuraminidase-treated human peripheral blood T-lymphocyte plasma membranes with affinity matrices comprising the peanut agglutinin co-valently immobilised on Sepharose 4B. This fraction could be specifically eluted from affinity columns using buffer solutions supplemented with 0.2 M D-galactose and was shown, by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (0.1%), to contain four major glycoprotein components with apparent molecular weights of 200000, 190000, 110000 and 95000. It is suggested, from the observed reactivity of these glycoproteins with various lectins in double-diffusion experiments, that they possess both O-glycosidically and N-glycosidically linked carbohydrates.