Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells
Effects of high glucose on expression of OPG and RANKL in rat aortic vascular smooth muscle cells
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高糖对大鼠主动脉血管平滑肌细胞OPG和RANKL表达的影响
DOI：
10.1016/s1995-7645(14)60317-5
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发表时间：
2015-03-01
影响因子：
3.1
通讯作者：
Zheng, Shao-Jiang
中科院分区：
文献类型：
--
作者：
Chang, Hong-Juan;Li, Tian-Fa;Zheng, Shao-Jiang
目的：探讨高糖对大鼠主动脉平滑肌细胞骨保护素（OPG）和核因子κ B受体激活因子配体（RANKL）表达的影响。方法：SD大鼠腹腔注射链脲佐菌素。免疫组化法检测大鼠胸主动脉OPG和RANKL的表达。在体外培养的血管平滑肌细胞（VSMCs）（A7 r5）中，应用yRT-PCR和Western blot检测OPG和RANKL的mRNA和蛋白水平。结果：高糖组大鼠主动脉VSMCs OPG表达增加，RANKL表达降低。此外，在体外实验中，高糖诱导OPG表达，但抑制RANKL表达的培养A7 r5的剂量和时间依赖性的方式。结论：结果提示，高糖可促进血管平滑肌细胞OPG的表达，抑制RANKL的表达，这可能是糖尿病血管钙化的分子机制之一。
Objective: To explore effect of high glucose on expression of osteoprotegerin (OPG) and receptor activator of NF-kappa B ligand (RANKL) in rat aortic vascular smooth muscle cells. Methods: SD rats were intraperitoneally injected with streptozotocin. OPG and RANKL expression in rat thoracic aortas were detected by immunohistochemical staining. In cultured vascular smooth muscle cells (VSMCs) (A7r5), yRT-PCR and Western blot analysis were used to examine the mRNA and protein levels of OPG and RANKL. Results: Our results demonstrated that OPG expression was increased in hyperglycemic rat aortic VSMCs, while RANKL expression was decreased. Besides, in vitro experiments high glucose induced OPG expression, but depressed RANKL expression by dose- and time-dependent manner in cultured A7r5. Conclusions: Our findings suggested that high glucose could promote the expression of OPG, and inhibit the expression of RANKL in VSMCs, which may be partly be the molecular mechanism of diabetic vascular calcification.