D1 dopamine receptor immunoreactivity in human and monkey cerebral cortex: predominant and extrasynaptic localization in dendritic spines.
D1 dopamine receptor immunoreactivity in human and monkey cerebral cortex: predominant and extrasynaptic localization in dendritic spines.
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DOI：
10.1073/pnas.91.12.5720
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发表时间：
1994-06
影响因子：
11.1
通讯作者：
J. Smiley;A. Levey;B. Ciliax;P. Goldman-Rakic
中科院分区：
文献类型：
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作者：
J. Smiley;A. Levey;B. Ciliax;P. Goldman-Rakic
在光镜和电镜下，D1多巴胺受体抗体在人和猴子大脑皮层的几个区域定位了该蛋白。除了猴子的细胞体标记外，人类和猴子的所有区域都有一个层流分布的neuropil标记，这是先前D1受体放射自显影研究预测的。在电镜下，在许多树突棘、树突轴和偶有的轴突末端可见到这种神经探针标记。虽然标记棘很常见，但它们只代表了所有皮质棘的一个子集。通过标记脊髓的连续切片显示，二氨基联苯胺反应产物通常不在突触后密度，而是移位到大的不对称（假定是谷氨酸能）突触的一侧。此外，大多数标记的脊髓没有接收到具有多巴胺能特征的突触，这表明许多D1受体位于突触外位点，可能通过神经细胞的扩散接收多巴胺。同样，双标记也未能揭示酪氨酸羟化酶免疫反应轴突突触上的D1标记。定位于许多树突棘表明D1受体的主要作用是调节谷氨酸能输入皮质锥体细胞。
Antibodies to the D1 dopamine receptor were used to localize this protein in several areas of human and monkey cerebral cortex with light and electron microscopy. In addition to cell body labeling in monkeys, all areas of humans and monkeys had a neuropil label with a laminar distribution predicted by previous D1 receptor autoradiography studies. Using electron microscopy, this neuropil label was seen in numerous dendritic spines, in dendritic shafts, and in occasional axon terminals. While labeled spines were common, they represented only a subset of all cortical spines. Serial sectioning through labeled spines showed that the diaminobenzidine reaction product was usually not at postsynaptic densities but instead was displaced to the side of the large asymmetric (presumed glutamatergic) synapse. Furthermore, most labeled spines did not receive synapses with dopaminergic features, suggesting that many D1 receptors are at extrasynaptic sites, possibly receiving dopamine via diffusion in the neuropil. Similarly, double labeling failed to reveal D1 labeling at synapses of tyrosine hydroxylase immunoreactive axons. Localization to numerous dendritic spines suggests that a primary role of D1 receptors is modulation of glutamatergic input to cortical pyramidal cells.