Expression of NDRG1, a differentiation-related gene, in human tissues
Expression of NDRG1, a differentiation-related gene, in human tissues
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DOI：
10.1007/s00418-002-0460-9
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发表时间：
2002-11-01
影响因子：
2.3
通讯作者：
Bosman, FT
中科院分区：
文献类型：
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作者：
Lachat, P;Shaw, P;Bosman, FT
NDRG I是新的N-myc下调基因（NDRG）家族的成员，属于α / β水解酶超家族，但没有水解催化位点。不同的生理和病理条件（缺氧、细胞分化、重金属、N-myc、肿瘤）可调节NDRG1的转录、mRNA的稳定性和翻译。在本报告中，我们在光镜和电镜下展示了NDRG1在大量正常人体组织中的免疫组织化学定位。NDRG1的免疫反应性多见于不同方面的上皮细胞。我们观察到NDRG1主要存在于细胞质中，但它也与细胞膜和粘附连接有关。鉴于NDRG1在缺氧条件下的强烈上调及其核定位，我们提出NDRG1在保护缺血细胞损伤中的作用。该蛋白的多个定位也表明其具有多种功能，其中包括参与e -钙粘蛋白/连环蛋白复合物的功能。
NDRG I is a member of the new N-myc down-regulated gene (NDRG) family which belongs to the alpha/beta hydrolase superfamily, but without presenting a hydrolytic catalytic site. Diverse physiological and pathological conditions (hypoxia, cellular differentiation, heavy metal, N-myc, neoplasia) modulate NDRG1 transcription, mRNA stability, and translation. In this report we present the immunohistochemical localization of NDRG1 in a large set of normal human tissues at light and electron microscopic levels. The immunoreactivity of NDRG1 is mostly found in epithelial cells with different aspects. We observed NDRG1 primarily in the cytoplasm, but it is also associated with the cellular membrane and adherens junctions. Given the strong upregulation of NDRG1 under hypoxia and its nuclear localization, we propose a role for NDRG1 in protection from ischernic cell damage. The multiple localizations of this protein also suggest pleiotropic functions amongst which a functional involvement in the E-cadherin/catenin complex.