Effects of Growth Hormone on Muscle and Bone in Female Mice: Role of Follistatin
Effects of Growth Hormone on Muscle and Bone in Female Mice: Role of Follistatin
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生长激素对雌性小鼠肌肉和骨骼的影响：卵泡抑素的作用
DOI：
10.1055/a-2003-5704
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发表时间：
2022
影响因子：
1.8
通讯作者：
Kaji Hiroshi
中科院分区：
文献类型：
--
作者：
Ohira Takashi;Kawao Naoyuki;Takafuji Yoshimasa;Mizukami Yuya;Kaji Hiroshi
肌肉和骨骼之间的相互作用在肌肉减少症和骨质疏松症的临床关系中得到了注意。骨骼肌分泌的肌因子在与各种生理和病理生理状态相关的肌-骨相互作用中发挥作用。尽管大量证据表明生长激素（GH）对肌肉和骨骼都有影响，但GH对肌肉-骨骼相互作用的影响仍然未知。因此，我们研究了生长激素给药8周对卵巢切除或未切除（OVX）小鼠肌肉和骨骼的影响，包括肌因子表达。生长激素显著增加了小鼠全身和下肢的肌肉质量，以及有或没有OVX的指长伸肌（EDL）和比目鱼肌的组织重量。此外，它还显著增加了两只老鼠的握力。对于股骨，GH显著增加了小鼠的皮质厚度和面积，无论有无OVX。此外，生长激素显著减弱了OVX小鼠骨小梁处骨体积与组织体积之比的下降。生长激素管理显著降低EDL中卵泡抑素mRNA水平，但没有影响有或没有OVX的小鼠的比目鱼肌，尽管它不影响所检查的其他肌因子。然而，生长激素显著提高小鼠血清卵泡素水平。总之，本研究表明，生长激素的使用增加了小鼠骨骼肌质量和握力，以及通过显微计算机断层扫描分析获得的皮质和骨小梁相关参数。然而，对于生长激素对肌肉和骨骼的影响，肌因子调节可能不是关键的。
The interactions between muscle and bone are noted in the clinical relationships between sarcopenia and osteoporosis. Myokines secreted from the skeletal muscles play roles in muscle-bone interactions related to various physiological and pathophysiological states. Although numerous evidence suggests that growth hormone (GH) influences both muscle and bone, the effects of GH on the muscle-bone interactions have remained unknown. We, therefore, investigated the influences of GH administration for 8 weeks on muscle and bone, including myokine expression, in mice with or without ovariectomy (OVX). GH administration significantly increased muscle mass in the whole body and lower limbs, as well as tissue weights of the extensor digitorum longus (EDL) and soleus muscles in mice with or without OVX. Moreover, it markedly increased grip strength in both mice. As for femurs, GH administration significantly increased cortical thickness and area in mice with or without OVX. Moreover, GH significantly blunted the decrease in the ratio of bone volume to tissue volume at the trabecular bone in mice with OVX. GH administration significantly decreased follistatin mRNA levels in the EDL, but not the soleus, muscles in mice with or without OVX, although it did not affect the other myokines examined. However, GH administration significantly elevated serum follistatin levels in mice. In conclusion, this study indicates that GH administration increases skeletal muscle mass and grip strength and cortical and trabecular bone-related parameters obtained by micro-computed tomography analyses in mice. However, myokine regulation might not be critical for the effects of GH on muscle and bone.