INTERPHASE CYTOGENETIC ANALYSIS OF ERBB2 AND TOPOII-ALPHA COAMPLIFICATION IN INVASIVE BREAST-CANCER AND POLYSOMY OF CHROMOSOME-17 IN DUCTAL CARCINOMA IN-SITU
INTERPHASE CYTOGENETIC ANALYSIS OF ERBB2 AND TOPOII-ALPHA COAMPLIFICATION IN INVASIVE BREAST-CANCER AND POLYSOMY OF CHROMOSOME-17 IN DUCTAL CARCINOMA IN-SITU
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DOI：
10.1002/ijc.2910640106
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发表时间：
1995-02-20
影响因子：
6.4
通讯作者：
KEITH, WN
中科院分区：
文献类型：
--
作者：
MURPHY, DS;MCHARDY, P;KEITH, WN
乳腺癌是一种基因复杂的疾病。荧光原位杂交可应用间期细胞遗传学分析乳腺癌进展的遗传学。我们分析了浸润性乳腺癌石蜡切片中erbB2和topoll α共扩增的组织学分布，并表明共扩增的位点在肿瘤中具有相同的组织学分布，并且在单个细胞核内具有相似的核分布。没有扩增的肿瘤区域很容易识别，并且可以将erbB2和topoll α共同扩增的肿瘤与仅erbB2扩增的肿瘤区分开来。此外，FISH用于显示乳腺非侵袭性导管原位癌中的17号染色体多体，以及乳腺癌活检中侵袭性和非侵袭性部分的erbB2扩增。这篇非侵袭性原位乳腺癌的间期细胞遗传学分析报告证明了FISH在乳腺癌进展的遗传学研究中的有用性。(C) 1995 Wiley-Liss, Inc。
Breast cancer is a genetically complex disease. Fluorescence in situ hybridisation can be used to analyse the genetics of breast-cancer progression by interphase cytogenetics. We have analysed the histological distribution of erbB2 and topoll alpha co-amplification in paraffin sections of invasive breast cancer and show that the co-amplified loci share the same histological distribution in the tumour and have a similar nuclear distribution within individual nuclei. Regions of the tumours without amplification are easily recognized and tumours with erbB2 and topoll alpha co-amplification can be distinguished from those with erbB2 amplification alone. In addition, FISH was used to show polysomy of chromosome 17 in non-invasive ductal carcinoma in situ of the breast and erbB2 amplification in both the invasive and non-invasive components of a breast cancer biopsy. This report of an interphase cytogenetic analysis of non-invasive breast carcinoma in situ demonstrates the usefulness of FISH for the genetic study of breast cancer progression. (C) 1995 Wiley-Liss, Inc.