Nisin, alone and combined with peptidoglycan-modulating antibiotics:: activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci
Nisin, alone and combined with peptidoglycan-modulating antibiotics:: activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci
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DOI：
10.1093/jac/dkf190
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发表时间：
2002-11-01
影响因子：
5.2
通讯作者：
Hamilton-Miller, JMT
中科院分区：
文献类型：
--
作者：
Brumfitt, W;Salton, MRJ;Hamilton-Miller, JMT
目的：我们寻求通过将nisin与其他已知的靶向细菌细胞壁生物合成的抗生素联合使用来规避耐药的方法。方法：采用标准化方法对耐甲氧西林金黄色葡萄球菌（MRSA）和耐万古霉素肠球菌（VRE）各20株进行体外单抗、联用杆菌肽、拉莫普宁和氯霉素的检测。结果：拉莫普兰活性最强，杆菌肽活性最低。nisin和ramoplanin相互作用。而氯霉素对乳酸链球菌素的活性有明显的拮抗作用。结论：观察nisin和ramoplanin对MRSA和VRE的协同作用，为nisin联合细胞壁肽聚糖抑制剂的概念提供了一个有希望的途径。为了使这种方法在临床上可行，还需要进一步的研究。
Objective: We have sought ways to circumvent resistance, by combining nisin with other antibiotics known to target bacterial cell wall biosynthesis.Methods: Twenty strains each of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) were tested in vitro by standardized methods against nisin alone and combined with bacitracin, ramoplanin and chloramphenicol.Results: Ramoplanin was the most potent compound, and bacitracin had the least activity. Two-way synergy was observed with nisin and ramoplanin. However, chloramphenicol was clearly antagonistic to the activity of nisin.Conclusions: Observations of synergy between nisin and ramoplanin against MRSA and VRE offer a promising approach to the concept of combining nisin with inhibitors of cell wall peptidoglycan. Further investigations are needed in order to develop this approach as a clinical possibility.