Stimulation of Cell Elongation by Tetraploidy in Hypocotyls of Dark-Grown Arabidopsis Seedlings.
Stimulation of Cell Elongation by Tetraploidy in Hypocotyls of Dark-Grown Arabidopsis Seedlings.
复制标题
DOI：
10.1371/journal.pone.0134547
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Nishitani K
中科院分区：
文献类型：
--
作者：
Narukawa H;Yokoyama R;Komaki S;Sugimoto K;Nishitani K
植物的大小很大程度上取决于单个细胞的大小。许多研究表明陆生植物的倍性和细胞大小之间存在联系，但这种联系仍然存在争议。本研究以拟南芥（Arabidopsis thaliana（L.）嘿最终下胚轴长度在四倍体植物比在二倍体植物，特别是当幼苗生长在黑暗中。四倍体幼苗下胚轴较长是由于细胞伸长增强而不是细胞数量增加所致。DNA微阵列分析表明，与二倍体下胚轴相比，参与角质层前体运输的基因在四倍体下胚轴的特定区域下调。甲苯胺蓝染色，角质层渗透性，评估和角质层结构，通过电子显微镜观察，改变四倍体植物。两者合计，这些数据表明，促进细胞伸长是负责在拟南芥下胚轴倍性依赖的大小决定，这一过程是直接或间接相关的角质层功能。
Plant size is largely determined by the size of individual cells. A number of studies showed a link between ploidy and cell size in land plants, but this link remains controversial. In this study, post-germination growth, which occurs entirely by cell elongation, was examined in diploid and autotetraploid hypocotyls of Arabidopsis thaliana (L.) Heynh. Final hypocotyl length was longer in tetraploid plants than in diploid plants, particularly when seedlings were grown in the dark. The longer hypocotyl in the tetraploid seedlings developed as a result of enhanced cell elongation rather than by an increase in cell number. DNA microarray analysis showed that genes involved in the transport of cuticle precursors were downregulated in a defined region of the tetraploid hypocotyl when compared to the diploid hypocotyl. Cuticle permeability, as assessed by toluidine-blue staining, and cuticular structure, as visualized by electron microscopy, were altered in tetraploid plants. Taken together, these data indicate that promotion of cell elongation is responsible for ploidy-dependent size determination in the Arabidopsis hypocotyl, and that this process is directly or indirectly related to cuticular function.