Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples
Establishment of the PCR system specific to Salmonella spp. and its application for the inspection of food and fecal samples
复制标题
DOI：
10.1292/jvms.61.1245
复制
发表时间：
1999-11-01
影响因子：
1.2
通讯作者：
Shirahata, T
中科院分区：
文献类型：
--
作者：
Makino, S;Kurazono, H;Shirahata, T
利用沙门氏菌肠毒素基因（Salmonella enterotoxin gene，RT-PCR）建立了沙门氏菌特异性PCR检测体系。分别用大豆肉汤和沙门氏菌增菌肉汤增菌，检出限为每克粪便和碎肉样品中1个细菌细胞。该PCR体系在公共卫生领域的实际应用中具有一定的实用价值。
We established the PCR detection system specific to Salmonella species using Salmonella enterotoxin gene (stn). The detection limit was one bacterial cell per one gram of fecal and minced-meat samples using enrichment procedure by Tripticase soy broth or Salmonella enrichment broth, respectively. We concluded that this PCR system is useful for the practical application in the field of the public hygiene.