Detection of potentially novel bacterial components of the human skin microbiota using culture-independent molecular profiling
Detection of potentially novel bacterial components of the human skin microbiota using culture-independent molecular profiling
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DOI：
10.1099/jmm.0.46075-0
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发表时间：
2005-12-01
影响因子：
3
通讯作者：
Benno, Y
中科院分区：
文献类型：
--
作者：
Dekio, I;Hayashi, H;Benno, Y
虽然形成皮肤微生物区系的微生物被认为在修饰和预防皮肤病方面发挥了重要作用，但由于难以确定样品中尚未培养的微生物，因此尚未对其组成进行全面分析。五名健康志愿者的擦拭前额皮肤样本通过分析16S rRNA基因和常规培养方法进行了分析，以提供皮肤微生物群的概况，其中包括来自正常皮肤的尚未培养的细菌。对16S rRNA基因序列的聚类分析表明，与培养方法相比，16S rRNA基因序列的多样性显著增加。通过对416个无性系的分析，得到19个已知种和13个新的系统模式。除表皮葡萄球菌和痤疮丙酸杆菌等常见细菌外，在5个样本中有3个样本检测到A型细菌，其中1个为优势克隆，其16S rRNA基因序列与甲基嗜甲氧菌的16S rRNA基因同源性为97%。用于检测人类皮肤微生物区系的16S rRNA基因独立于培养的遗传图谱使我们能够检测人类皮肤微生物区系中潜在的新成分。
Although the micro-organisms forming the cutaneous microbiota are considered to play important roles in the modification and prevention of skin diseases, a comprehensive analysis of their composition has not yet been carried out because of difficulties in determining yet-to-be-cultured micro-organisms in the samples. Swab-scrubbed forehead skin samples of five healthy volunteers were analysed by profiling 16S rRNA genes, as well as by conventional culture methods, to provide a profile of the cutaneous microbiota that included yet-to-be-cultured bacteria from normal human skin. Cluster analyses of the 16S rRNA gene sequences indicated a marked increase in diversity compared with that derived from the culture methods. Nineteen previously recognized species and 13 novel phylotypes were obtained from the analysis of 416 clones. In addition to well-known bacteria such as Staphylococcus epidermidis and Propionibacterium acnes, phylotype A, the 16S rRNA gene of which is 97% similar to that of Methylophilus methylotrophus, was detected in three of the five samples, in one of which it was the predominant clone. Culture-independent genetic profiling of 16S rRNA genes for detecting human cutaneous microbiota has allowed us to detect potentially novel components of the cutaneous microbiota in humans.