NONIDEALITY OF AQUEOUS-SOLUTIONS OF POLYETHYLENE-GLYCOL - CONSEQUENCES FOR ITS USE AS A MACROMOLECULAR CRYSTALLIZING AGENT IN VAPOR-DIFFUSION EXPERIMENTS
NONIDEALITY OF AQUEOUS-SOLUTIONS OF POLYETHYLENE-GLYCOL - CONSEQUENCES FOR ITS USE AS A MACROMOLECULAR CRYSTALLIZING AGENT IN VAPOR-DIFFUSION EXPERIMENTS
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DOI：
10.1107/s0907444995000436
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发表时间：
1995-09-01
期刊：
影响因子：
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通讯作者：
DETITTA, GT
中科院分区：
文献类型：
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作者：
ARAKALI, SV;LUFT, JR;DETITTA, GT
聚乙二醇（PEG 8000）与氯化钠，硫酸铵和硫酸镁七水合物的盐配对的浓度的微等压测量已经在坐滴安排与PEG在液滴和盐在水库。相对于水的蒸气压相等的PEG和盐的浓度的所得图是非线性的，不与它们的原点相交，并且证明相对低（mM）的盐浓度等同于相对高的PEG浓度。讨论了当采用PEG作为结晶剂时，通过气相扩散技术对大分子结晶的这些观察结果中的每一个的后果。
Microisopiestic measurements of the concentrations of polyethylene glycol (PEG 8000) paired with the salts sodium chloride, ammonium sulfate and magnesium sulfate heptahydrate have been made in a sitting-drop arrangement with PEG in the droplet and salt in the reservoir. Resulting graphs of the concentrations of PEG and salt that are equivalent with respect to the vapor pressure of water are non-linear, do not intersect their origins, and demonstrate that relatively low (mM) salt concentrations are equivalent to relatively high PEG concentrations. The consequences of each of these observations for macromolecular crystallization by the vapor-diffusion technique when PEG is employed as the crystallizing agent are discussed.