Genetic diversity, virulence genes and antimicrobial resistance of Salmonella Enteritidis isolated from food and humans over a 24-year period in Brazil
Genetic diversity, virulence genes and antimicrobial resistance of Salmonella Enteritidis isolated from food and humans over a 24-year period in Brazil
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DOI：
10.1016/j.fm.2012.06.008
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发表时间：
2012-12-01
影响因子：
5.3
通讯作者：
Falcao, Juliana P.
中科院分区：
文献类型：
--
作者：
Campioni, Fabio;Moratto Bergamini, Alzira Maria;Falcao, Juliana P.
沙门氏菌病是世界范围内的一个主要健康问题。在许多国家，血清型肠杆菌是沙门氏菌爆发的主要原因。在巴西，很少进行分子分型研究。本研究的目的是对在巴西分离的肠道沙门氏菌菌株进行分子分型，以确定食品和人类来源菌株之间的遗传关系，并评估其致病潜力和耐药性。共128个S。研究了1986年至2010年期间从人类粪便（67）和食物（61）中分离的肠道菌群。通过ERIC-PCR和使用XbaI的PFGE评估基因型多样性，通过纸片扩散法评估抗生素耐药性，并通过PCR评估SPI-1、SPI-2和pSTV毒力基因的存在。ERIC-PCR结果显示，112个菌株的相似性>85.4%; PFGE结果显示，96个菌株的相似性> 80.0%。几乎所有的菌株（97.6%）携带所有的13个毒力基因的调查。36株（28.12%）对萘啶酸耐药。总之，1996年后观察到的萘啶酸耐药性表明该药物的使用有所增加。这可能表明，这128个菌株可能来自一个共同的祖先，在24年内几乎没有什么不同，并且在巴西已经污染了食物和人类并导致疾病超过20年。(c)2012爱思唯尔有限公司保留所有权利。
Salmonellosis is a major health problem worldwide. Serovar Enteritidis has been a primary cause of Salmonella outbreaks in many countries. In Brazil, few molecular typing studies have been performed. The aims of this study were to molecularly type Salmonella Enteritidis strains isolated in Brazil in order to determine the genetic relationship between strains of food and human origin, as well as, to assess their pathogenic potential and antimicrobial resistance. A total of 128 S. Enteritidis strains isolated from human feces (67) and food (61) between 1986 and 2010 were studied. The genotypic diversity was assessed by ERIC-PCR and PFGE using Xbal, the antimicrobial resistance by the disc-diffusion assay and the presence of the SPI-1, SPI-2 and pSTV virulence genes assessed by PCR. The ERIC-PCR results revealed that 112 strains exhibited a similarity of >85.4% and the PFGE that 96 strains exhibited a similarity of >80.0%. Almost all strains (97.6%) harbored all 13 virulence genes investigated. Thirty-six strains (28.12%) were resistant to nalidixic acid. In conclusion, the nalidixic acid resistance observed after 1996 is indicative of an increase in the use of this drug. It may be suggested that these 128 strains might have descended from a common ancestor that differed little over 24 years and has been both contaminating food and humans and causing disease for more than two decades in Brazil. (c) 2012 Elsevier Ltd. All rights reserved.