Carbon nanotubes as intracellular protein transporters: Generality and biological functionality
Carbon nanotubes as intracellular protein transporters: Generality and biological functionality
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DOI：
10.1021/ja050062v
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发表时间：
2005-04-27
影响因子：
15
通讯作者：
Dai, HJ
中科院分区：
文献类型：
--
作者：
Kam, NWS;Dai, HJ
多种蛋白质会自发吸附在酸氧化单壁碳纳米管的侧壁上。这种简单的非特异性结合方式可用于制备非共价的蛋白质 - 纳米管复合物。研究发现，蛋白质能够借助纳米管通过内吞作用途径，很容易地被运输到各种哺乳动物细胞内部。一旦从内涵体中释放出来，内化的蛋白质 - 纳米管复合物能够进入细胞的细胞质并发挥生物学功能，运输的细胞色素c诱导细胞凋亡就证明了这一点。碳纳米管代表了一类新的分子运输载体，可能对未来的体外和体内蛋白质递送应用有用。
Various proteins adsorb spontaneously on the sidewalls of acid-oxidized single-walled carbon nanotubes. This simple nonspecific binding scheme can be used to afford noncovalent protein-nanotube conjugates. The proteins are found to be readily transported inside various mammalian cells with nanotubes acting as the transporter via the endocytosis pathway. Once released from the endosomes, the internalized protein-nanotube conjugates can enter into the cytoplasm of cells and perform biological functions, evidenced by apoptosis induction by transported cytochrome c. Carbon nanotubes represent a new class of molecular transporters potentially useful for future in vitro and in vivo protein delivery applications.