Chemotaxis away from thiocyanic and isothiocyanic esters in Pseudomonas aeruginosa.
Chemotaxis away from thiocyanic and isothiocyanic esters in Pseudomonas aeruginosa.
复制标题
铜绿假单胞菌中远离硫氰酸酯和异硫氰酸酯的趋化性。
DOI：
10.1111/j.1574-6968.1993.tb06488.x
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发表时间：
1993
影响因子：
2.1
通讯作者：
H. Ohtake
中科院分区：
文献类型：
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作者：
T. Ohga;Agus Masduki;Junichi Kato;H. Ohtake
负趋化性，生物体远离化学品的运动，在铜绿假单胞菌中使用快速录像带方法进行了研究。使用数字图像处理来检测含有测试化学品的毛细管口附近的细菌数量的变化。发现铜绿假单胞菌被硫氰酸酯和异硫氰酸酯排斥，包括异硫氰酸烯丙酯、硫氰酸乙酯、异硫氰酸甲酯和硫氰酸甲酯。特别是，细菌远离硫氰酸甲酯的运动是如此剧烈，以至于在开始观察后30 s内，毛细管口附近的细菌数量减少了约80%。突变株，完全能动，但缺乏积极的趋化性，没有逃脱的酯，这表明在这种生物体的积极和消极的趋化性之间的共同机制。
Negative chemotaxis, the movement of organisms away from chemicals, was investigated in Pseudomonas aeruginosa using a rapid videotape method. Digital image processing was used to detect changes in bacterial numbers near the mouth of a capillary containing a test chemical. P. aeruginosa was found to be repelled by thiocyanic and isothiocyanic esters including allyl isothiocyanate, ethyl thiocyanate, methyl isothiocyanate and methyl thiocyanate. Particularly, the movement of bacteria away from methyl thiocyanate was so drastic that bacterial numbers near the mouth of the capillary decreased by approximately 80% within 30 s after the start of observation. Mutant strains, fully motile but lacking positive chemotaxis, did not escape away from the esters, suggesting a common mechanism between positive and negative chemotaxes in this organism.
加藤淳一：“铜绿假单胞菌中的磷酸盐”J.Bacteriol。
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