Arabidopsis SEPALLATA proteins differ in cooperative DNA-binding during the formation of floral quartet-like complexes.
Arabidopsis SEPALLATA proteins differ in cooperative DNA-binding during the formation of floral quartet-like complexes.
复制标题
DOI：
10.1093/nar/gku755
复制
发表时间：
2014
影响因子：
14.9
通讯作者：
Melzer R
中科院分区：
文献类型：
--
作者：
Jetha K;Theißen G;Melzer R
拟南芥SEPALLATA(SEP)基因编码MADS结构域转录因子，决定了所有花器官的特性。四个拟南芥SEP基因的功能在很大程度上是多余的，但并不是完全冗余的。在这里，我们分析了SEP蛋白与DNA的相互作用。所有的蛋白质都能够在携带两个序列元件的DNA片段上形成四聚体四联体复合体，称为Carg-box。强协同结合DNA的Carg盒之间的距离在4-6个螺旋转弯的范围内。然而，SEP1也强烈地结合到间隔较小或较大距离的Carg盒对上，而SEP2倾向于大的结合，而SEP4倾向于结合较小的位点间距离。在所测试的大部分位点间距离中，SEP3的协同结合相对较弱。所有SEP蛋白都与靶基因AP3和SEP3上的花同源蛋白APETALA3(AP3)和雌蕊(PI)一起组成了花四联体复合体。我们的结果为解释为什么不同的SEP蛋白在决定花器官特性方面有很大但不是完全多余的功能提出了一个重要的部分：它们可能结合在很大程度上重叠的、但不是完全相同的靶基因集，这些靶基因在其顺式调节区中的Carg盒的排列和间距上不同。
The SEPALLATA (SEP) genes of Arabidopsis thaliana encode MADS-domain transcription factors that specify the identity of all floral organs. The four Arabidopsis SEP genes function in a largely yet not completely redundant manner. Here, we analysed interactions of the SEP proteins with DNA. All of the proteins were capable of forming tetrameric quartet-like complexes on DNA fragments carrying two sequence elements termed CArG-boxes. Distances between the CArG-boxes for strong cooperative DNA-binding were in the range of 4–6 helical turns. However, SEP1 also bound strongly to CArG-box pairs separated by smaller or larger distances, whereas SEP2 preferred large and SEP4 preferred small inter-site distances for binding. Cooperative binding of SEP3 was comparatively weak for most of the inter-site distances tested. All SEP proteins constituted floral quartet-like complexes together with the floral homeotic proteins APETALA3 (AP3) and PISTILLATA (PI) on the target genes AP3 and SEP3. Our results suggest an important part of an explanation for why the different SEP proteins have largely, but not completely redundant functions in determining floral organ identity: they may bind to largely overlapping, but not identical sets of target genes that differ in the arrangement and spacing of the CArG-boxes in their cis-regulatory regions.
登录
查看更多内容
影响因子：
64.5
作者：
JACK, T;BROCKMAN, LL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
64.5
作者：
DREWS, GN;BOWMAN, JL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
11.6
作者：
Huang, H;Tudor, M;Ma, H
通讯作者：
Ma, H
影响因子：
7.2
作者：
Castillejo, C;Romera-Branchat, M;Pelaz, S
通讯作者：
Pelaz, S
影响因子：
9.8
作者：
Kaufmann K;Muiño JM;Jauregui R;Airoldi CA;Smaczniak C;Krajewski P;Angenent GC
通讯作者：
Angenent GC