Initial study of microRNA expression profiles of colonic cancer without lymph node metastasis
Initial study of microRNA expression profiles of colonic cancer without lymph node metastasis
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DOI：
10.1111/j.1751-2980.2009.00413.x
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发表时间：
2010-02-01
影响因子：
3.5
通讯作者：
Gao, Heng Jun
中科院分区：
文献类型：
--
作者：
Wang, Ying Xin;Zhang, Xiao Yan;Gao, Heng Jun
目的：探讨无淋巴结转移的结肠癌与癌旁正常结肠组织中microRNA表达谱的差异，鉴定与结肠癌相关的特异性microRNA，并在此基础上预测microRNA的致癌机制。采用Agilent microRNA微阵列技术筛选microRNA的表达差异。使用特征提取软件分析数据。结果：共发现14种与结肠癌相关的miRNAs，其中miR-106 b、miR-135 b、miR-18 a、miR-18 b、miR-196 b、miR-19 a、miR-224、miR-335、miR-424、miR-20 a *、miR-301 b和miR-374 a在结肠癌组织中的表达较癌旁对照组上调，而miR-378和miR-378* 在结肠癌组织中的表达较癌旁对照组下调。结论：miR-18 a和miR-135 b在结肠癌组织和癌旁组织中存在差异表达。这些miRNAs中的许多被认为参与了多种肿瘤发生的过程。这些miRNAs可能在结肠癌的发生发展中起重要作用。这些结果为人类结直肠癌的发生提供了新的见解。
OBJECTIVE:To investigate the difference of microRNA expression profiles between colonic cancer without lymph node metastasis and the para-cancerous control, to identify the specific microRNA associated with the cancer and to predict the carcinogenetic mechanism of microRNA on the basis of these results.METHODS:The microRNA (miRNA) were extracted and isolated from six specimens, including colonic cancerous and para-cancerous ones, all of which were confirmed to be without lymph node metastasis. Agilent microRNA microarrays consisting of 723 probes were used for screening the expression differences of microRNA. Data were analyzed using feature extraction software. The expression level of differentially expressed microRNA using quantitative real-time polymerase chain reaction (RT-PCR) was validated.RESULTS:A total of 14 miRNAs were found to be associated with colonic cancer, in which the expression of miR-106b, miR-135b, miR-18a, miR-18b, miR-196b, miR-19a, miR-224, miR-335, miR-424, miR-20a*, miR-301b and miR-374a were up-regulated and the expression of miR-378 and miR-378* were downregulated in colonic cancer tissues, compared with the para-cancerous control. The expression level of miR-18a and miR-135b were validated in accordance with the results of RT-PCR.CONCLUSION:The miRNAs are differentially expressed between colonic tumor tissues and para-cancerous tissues. Many of these miRNAs are expected to participate in the process of multiple tumorigenesis. These miRNAs could play an important role in the carcinogenesis of colon. These results provide new insights in human colorectal cancer genesis.